Short-Chain Fatty Acids Modulate Sperm Migration through Olfactory Receptor 51E2 Activity
| Autor: | Emanuela Teveroni, Fiorella Di Nicuolo, Edoardo Vergani, Carmine Bruno, Giuseppe Maulucci, Giada Bianchetti, Anna Laura Astorri, Giuseppe Grande, Jacopo Gervasoni, Lavinia Santucci, Marco De Spirito, Andrea Urbani, Alfredo Pontecorvi, Francesca Mancini, Domenico Milardi |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Male
olfactory receptors OR51E2 short-chain fatty acids (SCFAs) sperm migration Organic Chemistry General Medicine Receptors Odorant Fatty Acids Volatile Spermatozoa Olfactory Receptor Neurons Catalysis Computer Science Applications Inorganic Chemistry Semen Animals Female Physical and Theoretical Chemistry Molecular Biology Spectroscopy |
| Zdroj: | International Journal of Molecular Sciences; Volume 23; Issue 21; Pages: 12726 |
| ISSN: | 1422-0067 |
| DOI: | 10.3390/ijms232112726 |
| Popis: | The non-orthotopic expression of olfactory receptors (ORs) includes the male reproductive system, and in particular spermatozoa; their active ligands could be essential to sperm chemotaxis and chemical sperm–oocyte communication. OR51E2 expression has been previously reported on sperm cells’ surface. It has been demonstrated in different cellular models that olfactory receptor 51E2 (OR51E2) binds volatile short-chain fatty acids (SCFAs) as specific ligands. In the present research, we make use of Western blot, confocal microscopy colocalization analysis, and the calcium-release assay to demonstrate the activation of sperm cells through OR51E2 upon SCFAs stimulus. Moreover, we perform a novel modified swim-up assay to study the involvement of OR51E2/SCFAs in sperm migration. Taking advantage of computer-assisted sperm analysis (CASA system), we determine the kinematics parameters of sperm cells migrating towards SCFAs-enriched medium, revealing that these ligands are able to promote a more linear sperm-cell orientation. Finally, we obtain SCFAs by mass spectrometry in cervico-vaginal mucus and show for the first time that a direct incubation between cervical mucus and sperm cells could promote their activation. This study can shed light on the possible function of chemosensory receptors in successful reproduction activity, laying the foundation for the development of new strategies for the treatment of infertile individuals. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |