Control of Calcium Oxalate Crystal Growth by Face-Specific Adsorption of an Osteopontin Phosphopeptide
| Autor: | DA Ionescu, Gilles A. Lajoie, Harvey A. Goldberg, Mej Mikko Karttunen, Bernd Grohe, Graeme K. Hunter, Jason O’Young, Kem A. Rogers |
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| Rok vydání: | 2007 |
| Předmět: |
Models
Molecular Phosphopeptides chemistry.chemical_classification Calcium Oxalate Chemistry Phosphopeptide Calcium oxalate General Chemistry Glutamic acid Biochemistry Catalysis Amino acid chemistry.chemical_compound Crystallography Colloid and Surface Chemistry Phosphoserine Phosphoprotein Biophysics Computer Simulation Osteopontin Adsorption Amino Acid Sequence Crystallization Peptide sequence Biomineralization |
| Zdroj: | Journal of the American Chemical Society, 129(48), 14946-14951. American Chemical Society |
| ISSN: | 1520-5126 0002-7863 |
| Popis: | Mineral-associated proteins have been proposed to regulate many aspects of biomineralization, including the location, type, orientation, shape, and texture of crystals. To understand how proteins achieve this exquisite level of control, we are studying the interaction between the phosphoprotein osteopontin (OPN) and the biomineral calcium oxalate monohydrate (COM). In the present study, we have synthesized peptides corresponding to amino acids 220-235 of rat bone OPN (pSHEpSTEQSDAIDpSAEK), one of several highly phosphorylated, aspartic-, and glutamic acid-rich sequences found in the protein. To investigate the role of phosphorylation in interaction with crystals, peptides containing no (P0), one (P1), or all three (P3) phosphates were prepared. Using a novel combination of confocal microscopy and scanning electron microscopy, we show that these peptides adsorb preferentially to {100} faces of COM and inhibit growth of these faces in a phosphorylation-dependent manner. To characterize the mechanism of adsorption of OPN peptides to COM, we have performed the first atomic-scale molecular-dynamics simulation of a protein-crystal interaction. P3 adsorbs to the {100} face much more rapidly than P1, which in turn adsorbs more rapidly than P0. In all cases, aspartic and glutamic acid, not phosphoserine, are the amino acids in closest contact with the crystal surface. These studies have identified a COM face-specific adsorption motif in OPN and delineated separate roles for carboxylate and phosphate groups in inhibition of crystal growth by mineral-associated phosphoproteins. We propose that the formation of close-range, stable, and face-specific interactions is a key factor in the ability of phosphoproteins to regulate biomineralization processes. |
| Databáze: | OpenAIRE |
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