An improved immunomagnetic procedure for the isolation of highly purified human blood eosinophils
| Autor: | T. Iff, C. Walker, S. Betz, Silvia Rihs, I J De Vries, M. Wandzilak, Kurt Blaser, T T Hansel |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Neutrophils
medicine.drug_class Immunology Eczema Cell Separation Receptors Fc Biology CD16 Granulocyte Monoclonal antibody Tetraspanin 29 Magnetics Antigen Antigens CD Superoxides medicine Humans Immunology and Allergy Membrane Glycoproteins Human blood Receptors IgG respiratory system Eosinophil Isolation (microbiology) Antigens Differentiation Molecular biology Eosinophils N-Formylmethionine Leucyl-Phenylalanine medicine.anatomical_structure Eosinophil chemotaxis |
| Zdroj: | Journal of Immunological Methods. 145:105-110 |
| ISSN: | 0022-1759 |
| DOI: | 10.1016/0022-1759(91)90315-7 |
| Popis: | A simple method is described for the isolation of human peripheral blood eosinophils by an immunomagnetic procedure. Superparamagnetic particles were coupled to a monoclonal antibody against CD16, a molecule present on neutrophils but not on eosinophils. A peripheral blood granulocyte preparation, containing neutrophils and eosinophils, was incubated with these anti-CD16 particles. In the magnetic field of a permanent magnet, magnetically labelled neutrophils were then retained on columns with a ferromagnetic matrix. By this negative selection procedure, eosinophils of 99.5% purity were obtained from normal individuals and 99.6% purity from patients with eczema. A comparison was made between the immunomagnetic method and eosinophil isolation after N-formyl-methionyl-leucyl-phenyl-alanine treatment. Even in the case of individuals with very low eosinophil counts, the immunomagnetic method permits the efficient isolation of highly purified and functionally active eosinophils. |
| Databáze: | OpenAIRE |
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