Validation of a competitive ELISA and a virus neutralization test for the detection and confirmation of antibodies to Senecavirus A in swine sera
| Autor: | Devi P. Patnayak, Charles K. Nfon, Melissa Goolia, Shawn Babiuk, Fabio A. Vannucci, Ming Yang |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Canada Screening test Swine Virus Neutralization Diagnostic Specificity Enzyme-Linked Immunosorbent Assay Picornaviridae Immunofluorescence Antibodies Viral Sensitivity and Specificity Serology 03 medical and health sciences Neutralization Tests parasitic diseases Medicine Animals Senecavirus A Swine Diseases Picornaviridae Infections General Veterinary biology medicine.diagnostic_test business.industry Antibodies Monoclonal Virology 030104 developmental biology biology.protein Antibody business Antibody detection |
| Zdroj: | Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc. 29(2) |
| ISSN: | 1943-4936 |
| Popis: | Senecavirus A (SVA; family Picornaviridae) is a nonenveloped, single-stranded RNA virus associated with idiopathic vesicular disease (IVD) in swine. SVA was detected in pigs with IVD in Brazil, United States, Canada, and China in 2015, triggering the need to develop and/or validate serologic assays for SVA. Our objective was to fully validate a previously developed competitive enzyme-linked immunosorbent assay (cELISA) as a screening test for antibodies to SVA. Additional objectives included the development and validation of a virus neutralization test (VNT) as a confirmatory test for SVA antibody detection, and the comparison of the cELISA, VNT, and an existing immunofluorescent antibody test (IFAT) for the detection of SVA antibodies in serial bleeds from SVA outbreaks. The diagnostic specificity and sensitivity were 98.2% (97.2–98.9%) and 96.9% (94.5–98.4%) for the cELISA, and 99.6% (99.0–99.9%) and 98.2% (95.8–99.4%) for the VNT, respectively. There was strong agreement among cELISA, VNT, and IFAT when compared based on kappa coefficient. Based on these performance characteristics, these tests are considered suitable for serologic detection of SVA in pigs. |
| Databáze: | OpenAIRE |
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