Acyl chain-dependent effect of lysophosphatidylcholine on endothelial prostacyclin production

Autor: Saša Frank, Wolfgang F. Graier, Akos Heinemann, Roland Malli, Gernot Desoye, Martin Hermansson, Andelko Hrzenjak, Horst Schweer, Michaela Tritscher, Pauli J. Ojala, Bernhard Watzer, Monika Riederer
Přispěvatelé: Department of Biochemistry and Developmental Biology
Rok vydání: 2010
Předmět:
Male
Endothelial lipase
Prostacyclin
030204 cardiovascular system & hematology
Phospholipase
Biochemistry
ACTIVATION
Mice
chemistry.chemical_compound
0302 clinical medicine
Endocrinology
311 Basic medicine
Research Articles
Cells
Cultured
0303 health sciences
CULTURED HUMAN
CYCLOOXYGENASE-2 EXPRESSION
cyclooxygenase
Lysophosphatidylcholine
endothelial cell
lipids (amino acids
peptides
and proteins)
Arachidonic acid
FATTY-ACIDS
medicine.drug
medicine.medical_specialty
education
LOW-DENSITY-LIPOPROTEIN
SIGNAL-TRANSDUCTION
QD415-436
Biology
ADHESION MOLECULES
03 medical and health sciences
PROTEIN-COUPLED RECEPTORS
Phospholipase A2
In vivo
Internal medicine
arachidonic acid
medicine
Animals
Humans
RNA
Messenger
prostanoids
030304 developmental biology
calcium
Lysophosphatidylcholines
Cell Biology
Epoprostenol
PHOSPHOLIPASE A(2)
Mice
Inbred C57BL
chemistry
Cyclooxygenase 2
CELLS
biology.protein
phospholipase A2
Endothelium
Vascular
Cyclooxygenase
Zdroj: Journal of Lipid Research, Vol 51, Iss 10, Pp 2957-2966 (2010)
ISSN: 0022-2275
Popis: Previously we identified palmitoyl-lysophosphatidylcholine (16:0 LPC), linoleoyl-LPC (18:2 LPC), arachidonoyl-LPC (20:4 LPC), and oleoyl-LPC (18:1 LPC) as the most prominent LPC species generated by the action of endothelial lipase (EL) on high-density lipoprotein. In the present study, the impact of those LPC on prostacyclin (PGI(2)) production was examined in vitro in primary human aortic endothelial cells (HAEC) and in vivo in mice. Although 18:2 LPC was inactive, 16:0, 18:1, and 20:4 LPC induced PGI(2) production in HAEC by 1.4-, 3-, and 8.3-fold, respectively. LPC-elicited 6-keto PGF1α formation depended on both cyclooxygenase (COX)-1 and COX-2 and on the activity of cytosolic phospholipase type IVA (cPLA2). The LPC-induced, cPLA2-dependent (14)C-arachidonic acid (AA) release was increased 4.5-fold with 16:0, 2-fold with 18:1, and 2.7-fold with 20:4 LPC, respectively, and related to the ability of LPC to increase cytosolic Ca(2+) concentration. In vivo, LPC increased 6-keto PGF(1α) concentration in mouse plasma with a similar order of potency as found in HAEC. Our results indicate that the tested LPC species are capable of eliciting production of PGI(2), whereby the efficacy and the relative contribution of underlying mechanisms are strongly related to acyl-chain length and degree of saturation.
Databáze: OpenAIRE
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