Characterization of oligosaccharides from the chondroitin sulfates
| Autor: | Thomas N. Huckerby, Stephen D. Weeks, Robert M. Lauder, Joanne Boocock, Gavin Brown, Karen M. Anderson, Ian A. Nieduszynski, Patricia L. Sandall |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Magnetic Resonance Spectroscopy
Stereochemistry Disaccharide Dermatan Sulfate Oligosaccharides Chondroitin ABC lyase Chondroitin ABC Lyase Disaccharides Biochemistry Dermatan sulfate chemistry.chemical_compound Sulfation Animals Chondroitin Tetrasaccharide Chondroitin sulfate Beta (finance) Chondroitin Lyases Chondroitin Sulfates Carbon Cartilage chemistry Cattle Protons Hydrogen |
| Zdroj: | European Journal of Biochemistry. 268:1181-1189 |
| ISSN: | 0014-2956 |
| DOI: | 10.1046/j.1432-1327.2001.01948.x |
| Popis: | Chondroitin sulfates were fragmented using the enzymes chondroitin sulfate ABC endolyase and chondroitin ACII lyase; both disaccharide and tetrasaccharide fragments were isolated after reduction to the corresponding 2-deoxy-2-N-acetylamino-D-galactitol (GalNAc-ol) form. These have the structures: Delta UA(beta 1--3)GalNAc4S-ol, Delta UA(beta 1--3)GalNAc6S-ol, Delta UA2S(beta 1--3)GalNAc6S-ol, Delta UA(beta 1--3)GalNAc4S(beta 1--4)L-IdoA(alpha 1--3)GalNAc4S-ol, Delta UA(beta 1--3)GalNAc4S(beta 1--4)GlcA(beta 1--3)GalNAc4S-ol, Delta UA(beta 1--3)GalNAc6S(beta 1--4)GlcA(beta 1--3)GalNAc4S-ol, Delta UA(beta 1--3)GalNAc6S(beta 1--4)GlcA(beta 1--3)GalNAc6S-ol, Delta UA2S(beta 1--3)GalNAc6S(beta 1--4)GlcA(beta 1--3)GalNAc4S-ol and Delta UA2S(beta 1--3)GalNAc6S(beta 1--4)GlcA(beta 1--3)GalNAc6S-ol, where Delta UA represents a 4,5-unsaturated hexuronic acid (4-deoxy-alpha-Lthreo-hex-4-enepyranosyluronic acid) and 6S/4S/2S represent O-ester sulfate groups at C6/C4/C2 sites. Complete (1)H-NMR and (13)C-NMR data are derived for these species, which may help to alleviate some of the significant difficulties resulting from signal complexity that are currently hindering the characterization and assignment of major and minor structural components within chondroitin sulfate and dermatan sulfate polymers. |
| Databáze: | OpenAIRE |
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