Absence of the mecC gene in methicillin-resistant Staphylococcus aureus isolated from various clinical samples: The first multi-centered study in Turkey
| Autor: | Faruk Karakeçili, Mehmet Parlak, Selahattin Atmaca, Bilge Gültepe, Serife Yuksekkaya, Aytekin Çikman, Ayşegül Çopur Çiçek, Fulya B. Bilman, Merve Aydin, Murat Kara, Muhammet Guzel Kurtoglu, Ihsan Hakki Ciftci, Baris Gulhan, Tuncer Özekinci |
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| Přispěvatelé: | SÜMBÜL, BİLGE, RTEÜ, Tıp Fakültesi, Dahili Tıp Bilimleri Bölümü, Çiçek, Ayşegül Çopur, Dicle Üniversitesi, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Tıbbi Mikrobiyoloji Ana Bilim Dalı, Özekinci, Tuncer, BAİBÜ, Tıp Fakültesi, Temel Tıp Bilimleri Bölümü, Kurtoğlu, Muhammet Güzel |
| Rok vydání: | 2019 |
| Předmět: |
Male
0301 basic medicine Turkey medicine.disease_cause physiological processes law.invention Methicillin 0302 clinical medicine law polycyclic compounds mecA 030212 general & internal medicine Child Polymerase chain reaction Aged 80 and over Geography lcsh:Public aspects of medicine General Medicine Middle Aged Staphylococcal Infections Anti-Bacterial Agents Intensive Care Units Infectious Diseases Staphylococcus aureus Child Preschool Female medicine.symptom medicine.drug Adult Methicillin-Resistant Staphylococcus aureus Adolescent 030106 microbiology The first multi-centered study in Turkey.- Journal of infection and public health cilt.12 ss.528-533 2019 [Cikman A. Aydin M. Gulhan B. Karakecili F. Kurtoglu M. Yuksekkaya S. Parlak M. Gultepe B. Cicek A. Bilman F. et al. -Absence of the mecC gene in methicillin-resistant Staphylococcus aureus isolated from various clinical samples] Microbial Sensitivity Tests Biology lcsh:Infectious and parasitic diseases Microbiology Young Adult 03 medical and health sciences Bacterial Proteins Intensive care Drug Resistance Bacterial medicine Humans Penicillin-Binding Proteins lcsh:RC109-216 Agar diffusion test Cefoxitin Aged mecC SCCmec Public Health Environmental and Occupational Health Infant lcsh:RA1-1270 Methicillin resistance biochemical phenomena metabolism and nutrition bacterial infections and mycoses Methicillin-resistant Staphylococcus aureus bacteria Sputum |
| Zdroj: | Journal of Infection and Public Health, Vol 12, Iss 4, Pp 528-533 (2019) |
| ISSN: | 1876-0341 |
| Popis: | KARA, Murat/0000-0002-4144-2409; aydin, merve/0000-0002-1522-6083 WOS: 000473414800014 PubMed: 30745200 Background: mecA is a predefined gene causing methicillin resistance in Staphylococcus aureus (S. aureus) isolates; however, it has been shown that some methicillin-resistant S. aureus (MRSA) strains do not carry this gene. Recently, in isolates found to be MRSA-positive but mecA-negative, a new resistance gene called mecC, which is a homolog of mecA, has been reported. This study aimed to investigate the mecC and mecA genes in MRSA strains isolated from different geographic regions in Turkey. Methods: the sample of the study consisted of 494 MRSA strains isolated from seven geographical regions in Turkey between 2013 and 2016. the strains were obtained from 17 centers, comprising 13 university hospitals, three education and research hospitals, and one state hospital. Methicillin resistance in S. aureus strains was determined using the agar disk diffusion method with a cefoxitin disk and the agar dilution method with oxacillin. the mecC and mecA genes in MRSA strains was investigated by Polymerase Chain Reaction (PCR). Results: of the MRSA strains investigated, 47.9% were isolated from intensive care units. Concerning sample type, 36.7% were detected in the respiratory tract (tracheal aspirate, sputum, etc.), 24.8% in blood, 18.7% in skin and soft tissues, 9.3% in nasal swabs, 5.4% in urine, 4.1% in ears, and 1% in sterile body fluid. Using PCR, mecC was not identified in any of the S. aureus strains isolated from different clinical microbiology laboratories. mecA gene positivity was found in 315 of the MRSA strains (63.8%). Staphylococcal Cassette Chromosome mec ( SCCmec) type was identified in 232 strains (46.9%), of which 136 (58.7%) were type II, 75 (32.4%) were type IV, 12 (5.1%) were type Mb, six (2.5%) were type I, and three (1.3%) were type III. Conclusion: This is the first multi-centered study to investigate MRSA strains isolated from different regions in Turkey. the mecC gene was not detected in any of the MRSA strains. We believe that this study will constitute an important basis for monitoring possible future changes. (C) 2019 the Authors. Published by Elsevier Limited on behalf of King Saud Bin Abdulaziz University for Health Sciences. Scientific Research and Project Unit of Erzincan Binali Yildirim University [SAG-A-240215-0121] This study was financially supported by the Scientific Research and Project Unit of Erzincan Binali Yildirim University (Project No: SAG-A-240215-0121). This research was presented as a poster in the 4th National Clinical Microbiology Congress in Antalya, Turkey, 2017. |
| Databáze: | OpenAIRE |
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