Salt tolerance response revealed by RNA-Seq in a diploid halophytic wild relative of sweet potato
| Autor: | Yan Luo, Robert W. Reid, Hengyou Zhang, Daniella Freese, Bao-Hua Song, Ann E. Loraine, Jonathan Watkins, Huazhong Shi, Changbao Li |
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| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Candidate gene Sequence analysis lcsh:Medicine Cellular homeostasis RNA-Seq Biology Plant Roots 01 natural sciences Article Transcriptome 03 medical and health sciences Stress Physiological lcsh:Science Gene Transcription factor 2. Zero hunger Multidisciplinary Sequence Analysis RNA Gene Expression Profiling lcsh:R food and beverages Molecular Sequence Annotation Salt Tolerance 15. Life on land Plant Leaves Gene expression profiling 030104 developmental biology Biochemistry Salts lcsh:Q Ipomoea Metabolic Networks and Pathways Signal Transduction 010606 plant biology & botany |
| Zdroj: | Scientific Reports, Vol 7, Iss 1, Pp 1-13 (2017) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Crop wild relatives harbor exotic and novel genetic resources, which hold great potential for crop improvement. Ipomoea imperati is a wild diploid relative of sweet potato with the capability of high salinity tolerance. We compared the transcriptomes of I. imperati under salt stress vs. control to identify candidate genes and pathways involved in salt response. De novo assembly produced 67,911 transcripts with a high depth of coverage. A total of 39,902 putative genes were assigned annotations, and 936 and 220 genes involved in salt response in roots and leaves, respectively. Functional analysis indicated a whole system response during salt stress in I. imperati, which included four metabolic processes: sensory initiation, transcriptional reprogramming, cellular protein component change, and cellular homeostasis regulation. We identified a number of candidate genes involved in the ABA signaling pathway, as well as transcription factors, transporters, antioxidant enzymes, and enzymes associated with metabolism of synthesis and catalysis. Furthermore, two membrane transporter genes, including vacuole cation/proton exchanger and inositol transporter, were considered to play important roles in salt tolerance. This study provided valuable information not only for understanding the genetic basis of ecological adaptation but also for future application in sweet potato and other crop improvements. |
| Databáze: | OpenAIRE |
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