Global transcriptional start site mapping inGeobacter sulfurreducensduring growth with two different electron acceptors
| Autor: | Leticia Vega-Alvarado, Enrique Morett, Maricela Olvera, Katy Juárez, Getzabeth González, Verónica Jiménez-Jacinto, Aurora Labastida |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Transcription Genetic Electrons Computational biology Biology Microbiology Genome Electron Transport 03 medical and health sciences Bacterial Proteins Genetics Transcriptional regulation Promoter Regions Genetic Molecular Biology Gene Geobacter sulfurreducens Regulation of gene expression Sequence Analysis RNA Gene Expression Profiling RNA Gene Expression Regulation Bacterial biology.organism_classification Gene expression profiling 030104 developmental biology Transcription Initiation Site Geobacter Genome Bacterial |
| Zdroj: | FEMS Microbiology Letters. 363:fnw175 |
| ISSN: | 1574-6968 |
| DOI: | 10.1093/femsle/fnw175 |
| Popis: | Geobacter sulfurreducens is an anaerobic soil bacterium that is involved in biogeochemical cycles of elements such as Fe and Mn. Although significant progress has been made in the understanding of the electron transfer processes in G. sulfurreducens , little is known about the regulatory mechanisms involved in their control. To expand the study of gene regulation in G. sulfurreducens , we carried out a genome-wide identification of transcription start sites (TSS) by 5′RACE and by deep RNA sequencing of primary mRNAs in two growth conditions. TSSs were identified along G. sulfurreducens genome and over 50% of them were located in the upstream region of the associated gene, and in some cases we detected genes with more than one TSS. Our global mapping of TSSs contributes with valuable information, which is needed for the study of transcript structure and transcription regulation signals and can ultimately contribute to the understanding of transcription initiation phenomena in G. sulfurreducens . |
| Databáze: | OpenAIRE |
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