In Vivo Effects of the Epstein–Barr Virus Small RNA EBER-1 on Protein Synthesis and Cell Growth Regulation
| Autor: | Ken Laing, Michael J. Clemens, Volker Matys, Vivienne J. Tilleray, Androulla Elia, B E Souberbielle, Ian W. Jeffrey |
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| Jazyk: | angličtina |
| Předmět: |
Herpesvirus 4
Human malignant transformation protein synthesis viruses Biology growth regulation Cell Line Malignant transformation Epstein–Barr virus Mice eIF-2 Kinase 03 medical and health sciences 0302 clinical medicine Neoplasms Virology hemic and lymphatic diseases fibroblasts Protein biosynthesis Animals Humans Cell Line Transformed 030304 developmental biology Regulation of gene expression 0303 health sciences Reporter gene HEK 293 cells RNA virus diseases protein kinase R Transfection Cell Transformation Viral Protein kinase R Molecular biology 3. Good health Gene Expression Regulation transfection EBER-1 RNA Protein Biosynthesis 030220 oncology & carcinogenesis tumourigenesis RNA Viral Cell Division |
| Zdroj: | Virology. (2):253-269 |
| ISSN: | 0042-6822 |
| DOI: | 10.1006/viro.2002.1354 |
| Popis: | Recent studies have suggested a role for the Epstein–Barr virus-encoded RNA EBER-1 in malignant transformation. EBER-1 inhibits the activity of the protein kinase PKR, an inhibitor of protein synthesis with tumour suppressor properties. In human 293 cells and murine embryonic fibroblasts, transient expression of EBER-1 promoted total protein synthesis and enhanced the expression of cotransfected reporter genes. However reporter gene expression was stimulated equally well in cells from control and PKR knockout mice. NIH 3T3 cells stably expressing EBER-1 exhibited a greatly increased frequency of colony formation in soft agar, and protein synthesis in these cells was relatively resistant to inhibition by the calcium ionophore A23187. Nevertheless clones containing a high concentration of EBER-1 were not invariably tumourigenic. We conclude that EBER-1 can enhance protein synthesis by a PKR-independent mechanism and that, although this RNA may contribute to the oncogenic potential of Epstein–Barr virus, its expression is not always sufficient for malignant transformation. |
| Databáze: | OpenAIRE |
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