Simple and effective serum-free medium for sustained expansion of bovine satellite cells for cell cultured meat
| Autor: | Stout, Andrew J., Mirliani, Addison B., Rittenberg, Miriam L., Shub, Michelle, White, Eugene C., Yuen Jr., John S. K., Kaplan, David L. |
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| Rok vydání: | 2022 |
| Předmět: |
Meat
low-cost serum-free media (B8) Medicine (miscellaneous) satellite cell expansion Cell Differentiation Culture Media Serum-Free General Biochemistry Genetics and Molecular Biology Myoblasts cultivated meat serum-free media cultured meat cellular agriculture Animals Cattle bovine satellite cells recombinant albumin General Agricultural and Biological Sciences serum Beefy-9 Cell Proliferation |
| Zdroj: | Communications Biology. 5 |
| ISSN: | 2399-3642 |
| Popis: | Cell-cultured meat offers the potential for a more sustainable, ethical, resilient, and healthy food system. However, research and development has been hindered by the lack of serum-free media that enable the robust expansion of relevant cells (e.g., muscle satellite cells) over multiple passages. Recently, a low-cost serum-free media (B8) was described for pluripotent stem cells. Here, B8 is adapted for bovine satellite cells through the addition of a single component, recombinant albumin, which renders it suitable for long-term satellite cell expansion without sacrificing myogenicity. This new media (Beefy-9) maintains cell growth over the entire period tested (seven passages), with an average doubling time of 39 h. Along with demonstrated efficacy for bovine cells, Beefy-9 offers a promising starting-point for developing serum-free media for other meat-relevant species. Ultimately, this work offers a foundation for escaping cultured meat research’s reliance on serum, thereby accelerating the field. |
| Databáze: | OpenAIRE |
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