Oral administration of the contact sensitizer trinitrochlorobenzene: initial sensitization and subsequent appearance of a suppressor population
| Autor: | Subhash C. Gautam, Jack R. Battisto, Nathaniel F. Chikkala |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Immunology
Population Administration Oral Spleen Picryl Chloride Administration Cutaneous Dermatitis Contact T-Lymphocytes Regulatory Mice Oral administration medicine Mesenteric lymph nodes Animals Antigens Ly education Cyclophosphamide Sensitization education.field_of_study Lamina propria Mice Inbred C3H biology Intestines medicine.anatomical_structure Phenotype biology.protein Female Antibody Hapten |
| Zdroj: | Cellular immunology. 125(2) |
| ISSN: | 0008-8749 |
| Popis: | Our earlier studies have demonstrated that intragastric administration of the hapten trinitrochlorobenzene (TNCB) 2 to 3 weeks prior to attempting sensitization with epidermally applied hapten can abrogate development of systemic contact sensitivity (CS). In this paper, we have examined whether onset of tolerance following intragastric administration of the hapten is preceded by development of hapten-specific CS. Indeed, CS was found to be present 5 days after feeding TNCB and in most experiments the response decreased significantly by Days 10 to 12. The kinetics of development of CS by the oral and epidermal routes were strikingly similar except that the magnitude of reactivity (up to 5 days) in orally sensitized mice was somewhat less than that of epidermally sensitized mice. With the exception of Peyer's patches (PP), effector cells of CS were recovered from such gut-associated lymphoid tissues as mesenteric lymph nodes (MLN), lamina propria, and lymphocytes that are present in the intraepithelial compartment of the intestinal wall. These cells as well as spleen cells of TNCB-fed mice were able to adoptively transfer CS to naive mice. The capacity of MLN and spleen cells of TNCB-fed mice to confer CS adoptively was abrogated after treating cells with anti-Thy 1.2 and anti-Lyt 1.1 antibodies plus complement thereby identifying them as T lymphocytes. Although CS decreased by 10–12 days after feeding TNCB, the decline was reversed by pretreating mice with cyclophosphamide (CY) 2 days before giving the hapten. Whereas spleen cells from animals fed hapten 5 days earlier transferred CS readily, those from mice fed hapten 12 days earlier did not. However, when 12-day spleen cells were depleted of Lyt 2 + cells their ability to adoptively transfer CS was restored. These observations indicate that feeding TNCB to mice initially produces CS, mediated by Thy 1.2 + , Lyt 1.1 + lymphocytes. CS is subsequently down-regulated by activation of Lyt 2 + suppressor cells, precursors of which are sensitive to CY. |
| Databáze: | OpenAIRE |
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