Paternal physical exercise modulates global DNA methylation status in the hippocampus of male rat offspring
| Autor: | Francele Valente Piazza, Matilde Achaval, Filipe Mega, Christiano Spindler, Viviane Rostirola Elsner, Ethiane Segabinazi, Simone Marcuzzo, André Luís Ferreira de Meireles, Gabriela dos Santos Salvalaggio |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
medicine.medical_specialty Offspring Exercício Morris water navigation task Hippocampus Herança paterna Physical exercise Biology Methylation sperm Metilação de DNA lcsh:RC346-429 03 medical and health sciences 0302 clinical medicine Developmental Neuroscience Internal medicine Fetal programming Neuroplasticity Hipocampo medicine Learning inheritance treadmill running Epigenetics Reelin nerve regeneration lcsh:Neurology. Diseases of the nervous system Inheritance learning epigenetics preconception Preconception Sperm methylation fetal programming Nerve regeneration 030104 developmental biology Endocrinology Treadmill running DNA methylation biology.protein 030217 neurology & neurosurgery Research Article |
| Zdroj: | Neural Regeneration Research, Vol 14, Iss 3, Pp 491-500 (2019) Neural Regeneration Research Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS |
| ISSN: | 1673-5374 |
| Popis: | It is widely known that maternal physical exercise is able to induce beneficial improvements in offspring cognition; however, the effects of paternal exercise have not been explored in detail. The present study was designed to evaluate the impact of paternal physical exercise on memory and learning, neuroplasticity and DNA methylation levels in the hippocampus of male offspring. Adult male Wistar rats were divided into two groups: sedentary or exercised fathers. The paternal preconception exercise protocol consisted of treadmill running, 20 minutes daily, 5 consecutive days per week for 22 days, while the mothers were not trained. After mating, paternal sperm was collected for global DNA methylation analysis. At postnatal day 53, the offspring were euthanized, and the hippocampus was dissected to measure cell survival by 5-bromo-2′-deoxiuridine and to determine the expression of synaptophysin, reelin, brain-derived neurotrophic factor and global DNA methylation levels. To measure spatial memory and learning changes in offspring, the Morris water maze paradigm was used. There was an improvement in spatial learning, as well as a significant decrease in hippocampal global DNA methylation levels in the offspring from exercised fathers compared with those from sedentary ones; however, no changes were observed in neuroplasticity biomarkers brain-derived neurotrophic factor, reelin and 5-bromo-2′-deoxiuridine. Finally, the global DNA methylation of paternal sperm was not significantly changed by physical exercise. These results suggest a link between paternal preconception physical activity and cognitive benefit, which may be associated with hippocampal epigenetic programming in male offspring. However, the biological mechanisms of this modulation remain unclear. |
| Databáze: | OpenAIRE |
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