Chemical composition and potentiating action of Norfloxacin mediated by the essential oil of Piper caldense C.D.C. against Staphylococcus aureus strains overexpressing efflux pump genes
Autor: | Luiz Everson da Silva, Emanuelle Machado Marinho, Antonio Linkoln Alves Borges Leal, Hélcio Silva dos Santos, Humberto Medeiros Barreto, Márcia Machado Marinho, Camila Fonseca Bezerra, Mayron Alves de Vasconcelos, Henrique Douglas Melo Coutinho, Emmanuel Silva Marinho, Terezinha Gonçalves da Silva, Camila Confortin, Alexandre Magno Rodrigues Teixeira |
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Jazyk: | angličtina |
Rok vydání: | 2021 |
Předmět: |
Methicillin-Resistant Staphylococcus aureus
Staphylococcus aureus Short Communication Microbial Sensitivity Tests medicine.disease_cause Antifungal Biochemistry Microbiology Essential oil law.invention 03 medical and health sciences Fungal dimorphism Bacterial Proteins law Genetics medicine Oils Volatile Candida albicans Molecular Biology Escherichia coli Norfloxacin 030304 developmental biology Efflux pump inhibitors 0303 health sciences biology 030306 microbiology Chemistry General Medicine Antimicrobial biology.organism_classification Corpus albicans Anti-Bacterial Agents Antibacterial Piper caldense Efflux Multidrug Resistance-Associated Proteins Piper medicine.drug |
Zdroj: | Archives of Microbiology |
ISSN: | 1432-072X 0302-8933 |
Popis: | Infectious diseases caused by multidrug-resistant microorganisms has increased in the last years. Piper species have been reported as a natural source of phytochemicals that can help in combating fungal and bacterial infections. This study had as objectives characterize the chemical composition of the essential oil from Piper caldense (EOPC), evaluate its potential antimicrobial activity, and investigate the synergistic effect with Norfloxacin against multidrug-resistant S. aureus overproducing efflux pumps, as well as, verify the EOPC ability to inhibit the Candida albicans filamentation. EOPC was extracted by hydrodistillation, and the chemical constituents were identified by gas chromatography, allowing the identification of 24 compounds (91.9%) classified as hydrocarbon sesquiterpenes (49.6%) and oxygenated sesquiterpenes (39.5%). Antimicrobial tests were performed using a 96-well plate microdilution method against C. albicans ATCC 10231, Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 standard strains, as well as against multidrug-resistant strains S. aureus SA1199B (overexpressing norA gene), S. aureus K2068 (overexpressing mepA gene) and S. aureus K4100 (overexpressing qacC gene). The oil showed activity against C. albicans ATCC 10231 (≥ 512 µg/mL) and was able to inhibit hyphae formation, an important mechanism of virulence of C. albicans. On the other hand, EOPC was inactive against all bacterial strains tested (≤ 1,024 µg mL). However, when combined with Norfloxacin at subinhibitory concentration EOPC reduced the Norfloxacin and Ethidium bromide MIC values against S. aureus strains SA1199B, K2068 and K4100. These results indicate that EOPC is a source of phytochemicals acting as NorA, MepA and QacC inhibitors. |
Databáze: | OpenAIRE |
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