Cytokines, growth, and environment factors in bone marrow plasma of acute lymphoblastic leukemia pediatric patients
| Autor: | Ester Mejstříková, Martina Vaskova, Vendula Pelkova, Jan Starý, Tomas Kalina, Denisa Petráčková, Jaroslav Weiser, Ondřej Hrušák, Markéta Žaliová, Martin Kovac |
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| Rok vydání: | 2014 |
| Předmět: |
Cell Survival
Clinical Biochemistry Immunology Protein Array Analysis Leukemia Inhibitory Factor Pathogenesis Bone Marrow In vivo Biomarkers Tumor medicine Humans Immunology and Allergy Cytokine Antibody RNA Messenger Child TIMP1 Tissue Inhibitor of Metalloproteinase-1 business.industry Precursor Cell Lymphoblastic Leukemia-Lymphoma Molecular medicine In vitro medicine.anatomical_structure Real-time polymerase chain reaction Cancer research Cytokines Bone marrow business |
| Zdroj: | European Cytokine Network. 25:8-13 |
| ISSN: | 1148-5493 |
| DOI: | 10.1684/ecn.2014.0348 |
| Popis: | Acute lymphoblastic leukemia (ALL) cells depend on the microenvironment of the host in vivo and do not survive in in vitro culture. Conversely, the suppression of non-malignant tissues is one of the leading characteristics of the course of ALL. Both the non-malignant suppression and malignant cell survival may be partly affected by soluble factors within the bone marrow (BM) environment. Here, we aimed to identify proteins in BM plasma of children with ALL that may contribute to ALL aggressiveness and/or the microenvironment-mediated survival of ALL cells. LBMp (leukemic bone marrow plasma) at the time of ALL diagnosis was compared to control plasma of bone marrow (CBMp) or peripheral blood (CPBp) using a cytokine antibody array. The cytokine antibody array enabled simultaneous detection of 79 proteins per sample. Candidate proteins exhibiting significantly different profiles were further analyzed and confirmed by ELISA. mRNA expression of one of the candidate proteins (TIMP1) was studied using quantitative reverse transcriptase polymerase chain reaction (qRTPCR). The cytokine antibody array experiments identified 23 proteins that differed significantly (p |
| Databáze: | OpenAIRE |
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