Drosophila ß
| Autor: | Nicole Pogodalla, Silke Rodrigues, Albert Cardona, Holger Kranenburg, Simone Rey, Christian Klämbt |
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| Přispěvatelé: | Cardona, Albert [0000-0003-4941-6536], Klämbt, Christian [0000-0002-6349-5800], Apollo - University of Cambridge Repository |
| Rok vydání: | 2020 |
| Předmět: |
Nervous system
Neuropil Science Central nervous system General Physics and Astronomy Nerve Tissue Proteins Blood–brain barrier General Biochemistry Genetics and Molecular Biology 38 38/1 Phosphatidylinositol Phosphates Cell polarity 38/89 medicine 631/378/1341 631/378/2596 Animals Drosophila Proteins Spectrin Cell Lineage Cytoskeleton Author Correction Glycoproteins Blood-brain barrier Neurons Multidisciplinary 631/378/87 Chemistry article Brain Glial biology General Chemistry Basolateral plasma membrane Cellular neuroscience Cell biology 64/24 medicine.anatomical_structure nervous system 38/35 Larva Drosophila Neuroglia |
| Zdroj: | Nature Communications Nature Communications, Vol 12, Iss 1, Pp 1-18 (2021) |
| ISSN: | 2041-1723 |
| Popis: | In the central nervous system (CNS), functional tasks are often allocated to distinct compartments. This is also evident in the Drosophila CNS where synapses and dendrites are clustered in distinct neuropil regions. The neuropil is separated from neuronal cell bodies by ensheathing glia, which as we show using dye injection experiments, contribute to the formation of an internal diffusion barrier. We find that ensheathing glia are polarized with a basolateral plasma membrane rich in phosphatidylinositol-(3,4,5)-triphosphate (PIP3) and the Na+/K+-ATPase Nervana2 (Nrv2) that abuts an extracellular matrix formed at neuropil-cortex interface. The apical plasma membrane is facing the neuropil and is rich in phosphatidylinositol-(4,5)-bisphosphate (PIP2) that is supported by a sub-membranous ßHeavy-Spectrin cytoskeleton. ßHeavy-spectrin mutant larvae affect ensheathing glial cell polarity with delocalized PIP2 and Nrv2 and exhibit an abnormal locomotion which is similarly shown by ensheathing glia ablated larvae. Thus, polarized glia compartmentalizes the brain and is essential for proper nervous system function. In the invertebrate CNS, synapses and dendrites are clustered in distinct neuropil areas that are separated from neuronal cell bodies by ensheathing glia (EG). Here, the authors show that Drosophila EG are polarized cells that form an internal diffusion barrier. EG cell polarity requires βHeavy-Spectrin and is needed for normal locomotor behaviour. |
| Databáze: | OpenAIRE |
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