Biophysical and biological activity of a synthetic 8.7-kDa hydrophobic pulmonary surfactant protein SP-B
| Autor: | B L Ohning, T K Leung, Virender Kumar Sarin, J L Fox, V E Taylor, S Gupta, Jeffrey A. Whitsett |
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| Rok vydání: | 1990 |
| Předmět: |
Proteolipids
Molecular Sequence Data Peptide Hydrofluoric Acid Wilhelmy balance Pulmonary surfactant medicine Animals Humans Surface Tension Amino Acid Sequence RNA Messenger Peptide sequence Lung Antiserum chemistry.chemical_classification Multidisciplinary Chemistry Immune Sera Biological activity Pulmonary Surfactants medicine.anatomical_structure Biochemistry Cattle Electrophoresis Polyacrylamide Gel Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 87(7) |
| ISSN: | 0027-8424 |
| Popis: | We have synthesized pulmonary surfactant apoprotein SP-B peptides by solid-phase chemistry and demonstrated their ability to enhance the surface-active properties of synthetic lipid mixtures. The synthetic peptides were reactive with antiserum generated against the native bovine surfactant peptide. Both peptides conferred surfactant-like properties to synthetic lipid mixtures as assessed by a Wilhelmy balance and pulsating bubble surfactometer. Likewise, mixtures of synthetic SP-B peptides and lipid restored compliance of isolated surfactant-deficient rat lungs. This work demonstrates the utility of SP-B as a functional component of pulmonary surfactant mixtures for treatment of respiratory distress syndrome or other disorders characterized by surfactant deficiency. |
| Databáze: | OpenAIRE |
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