Heat shock protein-peptide complexes elicit cytotoxic T-lymphocyte and antibody responses specific for bovine herpesvirus 1
| Autor: | Muralidhar S. Deshpande, Manjula Navaratnam, Douglas S. Zatechka, Mangala J Hariharan, S. Srikumaran |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Cellular immunity
animal diseases viruses Antigen presentation chemical and pharmacologic phenomena In Vitro Techniques Antibodies Viral Epitope Epitopes Mice Viral Proteins Antigens Neoplasm Animals Cytotoxic T cell Amino Acid Sequence HSP90 Heat-Shock Proteins Antigens Viral Herpesvirus 1 Bovine Mice Inbred BALB C General Veterinary General Immunology and Microbiology biology MHC class I antigen Immunogenicity H-2 Antigens Public Health Environmental and Occupational Health hemic and immune systems biochemical phenomena metabolism and nutrition biology.organism_classification Virology Bovine herpesvirus 1 CTL Infectious Diseases Molecular Medicine Cattle Female T-Lymphocytes Cytotoxic |
| Zdroj: | Vaccine. 19:1425-1434 |
| ISSN: | 0264-410X |
| DOI: | 10.1016/s0264-410x(00)00381-9 |
| Popis: | Epitope-based vaccines offer a promising alternative to modified live vaccines against viruses such as herpesviruses which give rise to latent infections, and induce immunosuppression. The success of this approach depends on the ability to direct the CTL epitopes to the MHC class I antigen presentation pathway. The objective of this study was to evaluate the potential of the heat shock protein gp96 in this regard. A group of BALB/c mice was injected with three murine CTL epitope peptides of bovine herpesvirus 1 (BHV-1) complexed in vitro with bovine gp96 (gp96-peptides). Three other groups were injected with either the peptides alone, gp96 alone, or the peptides complexed with BSA. CTLs from mice immunized with gp96-peptides specifically lysed the peptide-pulsed syngeneic targets, as well as BHV-1-infected targets. CTLs from the other three groups did not lyse these targets. To further evaluate the utility of this approach, groups of BALB/c mice were immunized with gp96 isolated from a syngeneic cell-line transduced with BHV-1 glycoprotein D (BC-gD). Mice immunized with gp96 from BC-gD developed CTLs, as well as Abs specific for BHV-1 gD. Furthermore, in vitro stimulation of naive bovine PBMCs with gp96 from BC-gD resulted in CTLs specific for BHV-1. These results demonstrate the feasibility of using gp96-peptide complexes isolated from cells expressing BHV-1 proteins to induce CTL and Ab responses against BHV-1, without the prior knowledge of the CTL and Ab epitope sequences. |
| Databáze: | OpenAIRE |
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