Immunoblot and radioallergosorbent test inhibition studies of allergenic cross-reactivity of the predatory mite Amblyseius cucumeris with the house dust mite Dermatophagoides pteronyssinus
| Autor: | Ronald van Ree, A. M. Vermeulen, G. C. M. Groenewoud, Nicolette W. de Jong, Astrid van Leeuwen, Albert W. Van Toorenenbergen, Roy Gerth van Wijk, Hans de Groot |
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| Přispěvatelé: | Experimental Immunology |
| Jazyk: | angličtina |
| Rok vydání: | 2004 |
| Předmět: |
Hypersensitivity
Immediate Male Dermatophagoides pteronyssinus Acariformes medicine.disease_cause Trees Cohort Studies Allergen immune system diseases Immunology and Allergy Netherlands Skin education.field_of_study Mites biology medicine.diagnostic_test Radioallergosorbent test Middle Aged Pollen Female Capsicum Pulmonary and Respiratory Medicine Adult Phytoseiidae Immunology Population Blotting Western Cross Reactions Poaceae Microbiology Dogs Radioallergosorbent Test medicine Mite Respiratory Hypersensitivity Animals Humans education Skin Tests House dust mite Tissue Extracts Pyroglyphidae Allergens Immunoglobulin E biology.organism_classification Agricultural Workers' Diseases respiratory tract diseases Cats Hair |
| Zdroj: | Annals of allergy, asthma & immunology, 93(3), 281-287. American College of Allergy, Asthma and Immunology |
| ISSN: | 1081-1206 |
| DOI: | 10.1016/s1081-1206(10)61502-5 |
| Popis: | BACKGROUND: In 1999, an extensive study among bell pepper growers showed that a predatory mite, Amblyseius cucumeris, is a potentially relevant source of occupational allergens because 23% of the population had positive skin prick test reactions. OBJECTIVE: To investigate whether cross-reactivity between A. cucumeris and Dermatophagoides pteronyssinus is responsible for the cosensitization to both mite species found in 58.7% of A. cucumeris-sensitized greenhouse workers. METHODS: Fifteen serum samples from greenhouse workers with work-related inhalant allergy and a positive radioallergosorbent test (RAST) reaction to A. cucumeris or D. pteronyssinus were selected for immunoblot analysis using extracts of both mites. A subselection (n = 5) was used for RAST and immunoblot inhibition to investigate potential cross-reactivity. RESULTS: On immunoblot, 2 distinct patterns were observed: one pattern showed common protein bands in A. cucumeris and D. pteronyssinus blots suggestive of cross-reactivity between A. cucumeris and D. pteronyssinus and the other pattern showed no shared protein bands. Dermatophagoides pteronyssinus RAST inhibition with A. cucumeris extract was low in 4 serum samples ( |
| Databáze: | OpenAIRE |
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