Skeletal muscle specification by myogenin and Mef2D via the SWI/SNF ATPase Brg1
| Autor: | Concetta G.A. Marfella, Yasuyuki Ohkawa, Anthony N. Imbalzano |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Histone Deacetylase 2
Biology MyoD Muscle Development General Biochemistry Genetics and Molecular Biology Article Histone Deacetylases Cell Line Mice MyoD Protein Adenosine Triphosphate medicine Animals Muscle Skeletal Molecular Biology Myogenin General Immunology and Microbiology Myogenesis MEF2 Transcription Factors General Neuroscience DNA Helicases Skeletal muscle Gene Expression Regulation Developmental Nuclear Proteins Cell Differentiation musculoskeletal system Chromatin Assembly and Disassembly Molecular biology SWI/SNF Mice Inbred C57BL Repressor Proteins medicine.anatomical_structure Myogenic Regulatory Factors Myogenic regulatory factors Ectopic expression Female Myogenic Regulatory Factor 5 tissues Transcription Factors |
| Popis: | Myogenin is required not for the initiation of myogenesis but instead for skeletal muscle formation through poorly understood mechanisms. We demonstrate in cultured cells and, for the first time, in embryonic tissue, that myogenic late genes that specify the skeletal muscle phenotype are bound by MyoD prior to the initiation of gene expression. At the onset of muscle specification, a transition from MyoD to myogenin occurred at late gene loci, concomitant with loss of HDAC2, the appearance of both the Mef2D regulator and the Brg1 chromatin-remodeling enzyme, and the opening of chromatin structure. We further demonstrated that ectopic expression of myogenin and Mef2D, in the absence of MyoD, was sufficient to induce muscle differentiation in a manner entirely dependent on Brg1. These results indicate that myogenin specifies the muscle phenotype by cooperating with Mef2D to recruit an ATP-dependent chromatin-remodeling enzyme that alters chromatin structure at regulatory sequences to promote terminal differentiation. |
| Databáze: | OpenAIRE |
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