Retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) restore the germline competence of in vitro cultured chicken blastodermal cells
| Autor: | Ning Xiao, Hao Hu, Fengshuo Yang, Jun Shi, Xiaochuan Tang, Xiaoli Wang, Deshun Shi, Xiaolian Qin, Rongyang Li |
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| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
animal structures Green Fluorescent Proteins Retinoic acid Tretinoin Bone Morphogenetic Protein 4 Chick Embryo Biology Germline Animals Genetically Modified 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine medicine Animals Blastoderm Induced pluripotent stem cell Gene Cells Cultured Gene Expression Regulation Developmental Cell Differentiation Embryo Cell Biology General Medicine Recombinant Proteins In vitro Cell biology 030104 developmental biology medicine.anatomical_structure Bone morphogenetic protein 4 chemistry 030220 oncology & carcinogenesis embryonic structures Germ cell Signal Transduction Developmental Biology |
| Zdroj: | In Vitro Cellular & Developmental Biology - Animal. 55:169-176 |
| ISSN: | 1543-706X 1071-2690 |
| DOI: | 10.1007/s11626-019-00324-9 |
| Popis: | Chicken blastodermal cells (BCs) are pluripotent stem cells derived from early embryos and may be easily obtained and manipulated. However, in vitro cultured BCs have extremely low germline capacity, which may limit their applications. Research on the germ cell differentiation of mammalian pluripotent cells using chemical-inducing agents has gained popularity, and tremendous achievements have been made. Whether chemical-inducing agents allow acquirement of germline competence in BCs is, however, questionable. In this study, retinoic acid (RA) and bone morphogenetic protein 4 (BMP4) promoted the expression of germline-specific genes and restored the germline competence of in vitro cultured BCs. Moreover, BCs induced with RA and BMP4 could efficiently produce gonadal chimeric chick embryos. These results may greatly enhance the potential applications of BCs in biotechnology and basic research. |
| Databáze: | OpenAIRE |
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