The effect of exposure to 1800 MHz radiofrequency radiation on epidermal growth factor, caspase-3, Hsp27 and p38MAPK gene expressions in the rat eye
| Autor: | Nurcan Aras, Badel Arslan, Metin Yildirim, Ebru Derici Eker, Ayşegül Akar |
|---|---|
| Přispěvatelé: | OMÜ |
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Economics and Econometrics
radiofrequency radiation caspase-3 animal structures DNA damage HSP27 Heat-Shock Proteins Gene Expression Caspase 3 medicine.disease_cause Eye p38 Mitogen-Activated Protein Kinases 03 medical and health sciences 0302 clinical medicine Hsp27 Epidermal growth factor Heat shock protein Gene expression Materials Chemistry Media Technology medicine Animals Epidermal growth factor receptor Rats Wistar rat eye biology Epidermal Growth Factor Chemistry Electromagnetic Radiation Forestry Molecular biology 030220 oncology & carcinogenesis biology.protein gene expression p38MAPK RNA Female 030217 neurology & neurosurgery Oxidative stress |
| DOI: | 10.4149/BLL_2018_106 |
| Popis: | WOS: 000447661800010 PubMed: 30226071 OBJECTIVE: Radiofrequency electromagnetic fields (RF-EMF) may induce DNA damage and oxidative stress in human lens epithelial cells (LECs). We aimed to investigate the expression levels of heat shock protein 27 (Hsp27), p38 mitogen-activated protein kinase (p38MAPK), epidermal growth factor receptor (EGFR) and caspase-3 gene expression levels in rat eye that was exposed to 1800 MHz RF-EMF. METHODS: Thirty-seven female Wistar albino rats were divided into three groups. The rats in the study group (n = 9) were exposed to 1800 MHz RF-EMF at an electric field 6.8 +/- 0.1 V/m and 0.06 W/kg specific absorption rate (SAR) for 2 hours per day for eight weeks. Sham group (n = 9) was kept under similar conditions as the exposed group without exposure to RF-EMF. The rats in all three groups were sacrificed and their eyes were removed. Hsp27, p38MAPK, EGFR, caspase-3 gene expression levels were investigated in detail with real-time polymerase chain reactions (Real-Time PCR). RESULTS: caspase-3 and p38MAPK gene expression were significantly upregulated in the ocular tissues following exposure to RF-EMF (p < 0.05). CONCLUSION: According to our findings, eye cells recognize EMF as a stress factor, and in response, activate caspase-3 and p38MAPK gene expressions. These results confirm that RF-EMF can cause cellular damage in rat ocular cells (Tab. 2, Fig. 3, Ref. 37). Text in PDF www.elis.sk. Department of Scientific Research Projects, Mersin University [BAP-SBE TBB(BAM) 2014-1 DR] This work was supported by the Department of Scientific Research Projects, Mersin University (Grant Number BAP-SBE TBB(BAM) 2014-1 DR).mmm |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|