Introducing extra NADPH consumption ability significantly increases the photosynthetic efficiency and biomass production of cyanobacteria
| Autor: | Fuliang Zhang, Yanping Zhang, Yin Li, Hengkai Meng, Jie Zhou |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Cyanobacteria Light Photosystem II Energy balance Biomass Bioengineering Photosynthetic efficiency Photosynthesis Photosystem I Applied Microbiology and Biotechnology Metabolic engineering 03 medical and health sciences Botany Cell Proliferation biology biology.organism_classification Genetic Enhancement 030104 developmental biology Metabolic Engineering Biophysics Metabolic Networks and Pathways NADP Biotechnology |
| Zdroj: | Metabolic Engineering. 38:217-227 |
| ISSN: | 1096-7176 |
| Popis: | Increasing photosynthetic efficiency is crucial to increasing biomass production to meet the growing demands for food and energy. Previous theoretical arithmetic analysis suggests that the light reactions and dark reactions are imperfectly coupled due to shortage of ATP supply, or accumulation of NADPH. Here we hypothesized that solely increasing NADPH consumption might improve the coupling of light reactions and dark reactions, thereby increasing the photosynthetic efficiency and biomass production. To test this hypothesis, an NADPH consumption pathway was constructed in cyanobacterium Synechocystis sp. PCC 6803. The resulting extra NADPH-consuming mutant grew much faster and achieved a higher biomass concentration. Analyses of photosynthesis characteristics showed the activities of photosystem II and photosystem I and the light saturation point of the NADPH-consuming mutant all significantly increased. Thus, we demonstrated that introducing extra NADPH consumption ability is a promising strategy to increase photosynthetic efficiency and to enable utilization of high-intensity lights. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect