The use of mGnRHa provokes ovulation but not viable embryos in Leporinus macrocephalus
| Autor: | Renata Guimarães Moreira, Thiago Scremin Boscolo Pereira, Camila Nomura Pereira Boscolo, Sergio Ricardo Batlouni |
|---|---|
| Přispěvatelé: | Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP) |
| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Ovulation medicine.medical_specialty Metoclopramide FISIOLOGIA ANIMAL Spawning performance media_common.quotation_subject Aquatic Science Biology Andrology Final maturation 03 medical and health sciences Internal medicine medicine Carp media_common Embryo biology.organism_classification Gonadal steroids 030104 developmental biology Endocrinology Hormonal treatment Reproduction Leporinus macrocephalus Agronomy and Crop Science Tropical fish Hormone medicine.drug |
| Zdroj: | Scopus Repositório Institucional da UNESP Universidade Estadual Paulista (UNESP) instacron:UNESP Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| Popis: | Made available in DSpace on 2018-12-11T17:04:46Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-04-01 Fundação de Amparo à Pesquisa do Estado de Goiás In rheophilic tropical fish, the use of carp pituitary extract (CPE) is still the main protocol since the use of gonadotropin-releasing hormone (GnRH) induces ovulation, but usually does not provide viable embryos. Thus, in order to generate knowledge to support the establishment of a successful protocol using GnRH, we compared the ovulatory process and reproductive performance of Leporinus macrocephalus using CPE (0.5 and 5.0 mg kg−1) and mGnRHa (7 µg kg−1) associated with metoclopramide. Females of both treatments ovulated, but the mGnRHa treatment did not provide viable embryos, which was associated with a more potent ovulation, lower latency period and less intense hydration of eggs, suggesting a possible treatment overstimulation. This is the first report associating a less intense egg hydration process to a failure in obtaining viable eggs in induced fish spawning. Considering that the mGnRHa doses applied here were quite low, it is possible that L. macrocephalus as well as other rheophilic tropical species present a higher sensibility to mGnRHa and/or metoclopramide (or derived substances) and future approaches must consider using even lower doses of these substances as well as testing different types of GnRH and dopamine inhibitors. Centro de Aquicultura Universidade Estadual Paulista (UNESP), Campus de Jaboticabal Instituto de Biociências Universidade de São Paulo (USP), Campus de São Paulo Centro de Aquicultura Universidade Estadual Paulista (UNESP), Campus de Jaboticabal Fundação de Amparo à Pesquisa do Estado de Goiás: 2010/08334-7 |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|