The mitochondrial translocase of the inner membrane PaTim54 is involved in defense response and longevity in Podospora anserina

Autor: Philippe Silar, Sylvain Brun, Alex Mercier, David Morais, Robert Debuchy, Colin Clairet
Přispěvatelé: Laboratoire Interdisciplinaire des Energies de Demain (LIED (UMR_8236)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Meiotic Recombination and Pairing (MRP), Département Biologie des Génomes (DBG), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Institut de Biologie Intégrative de la Cellule (I2BC), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Universite Paris-Diderot intramural funding, BIOlogie et GEstion des Risques en agriculture (BIOGER), AgroParisTech-Institut National de la Recherche Agronomique (INRA), Différenciation sexuée et méiose chez les champignons (DSMC), Institut de génétique et microbiologie [Orsay] (IGM), Université Paris-Sud - Paris 11 (UP11)-Centre National de la Recherche Scientifique (CNRS)
Rok vydání: 2019
Předmět:
Zdroj: Fungal Genetics and Biology
Fungal Genetics and Biology, 2019, 132 (103257), pp.103257. ⟨10.1016/j.fgb.2019.103257⟩
Fungal Genetics and Biology, Elsevier, 2019, 132, pp.103257. ⟨10.1016/j.fgb.2019.103257⟩
Fungal Genetics and Biology, Elsevier, 2019, 132 (103257), pp.103257. ⟨10.1016/j.fgb.2019.103257⟩
ISSN: 1087-1845
1096-0937
DOI: 10.1016/j.fgb.2019.103257
Popis: International audience; Fungi are very successful microorganisms capable of colonizing virtually any ecological niche where they must constantly cope with competitors including fungi, bacteria and nematodes. We have shown previously that the ascomycete Podopora anserina exhibits Hyphal Interference (HI), an antagonistic response triggered by direct contact of competing fungal hyphae. When challenged with Penicillium chrysogenum, P. anserina produces hydrogen peroxide at the confrontation and kills the hyphae of P. chrysogenum. Here, we report the characterization of the PDC2218 mutant affected in HI. When challenged with P. chrysogenum, the PDC2218 mutant produces a massive oxidative burst at the confrontation. However, this increased production of hydrogen peroxide is not correlated to increased cell death in P. chrysogenum. Hence, the oxidative burst and cell death in the challenger are uncoupled in PDC2218. The gene affected in PDC2218 is PaTim54, encoding the homologue of the budding yeast mitochondrial inner membrane import machinery component Tim54p. We show that PaTim54 is essential in P. anserina and that the phenotypes displayed by the PDC2218 mutant, renamed PaTim542218, are the consequence of a drastic reduction in the expression of PaTim54. Among these pleiotropic phenotypes, PDC2218-PaTim542218- displays increased lifespan, a phenotype in line with the observed mitochondrial defects in the mutant.
Databáze: OpenAIRE
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