Prevascular Structures Promote Vascularization in Engineered Human Adipose Tissue Constructs Upon Implantation
Autor: | Sandra J. Posthumus-van Sluijs, Steven E.R. Hovius, Gerjo J.V.M. van Osch, Femke Verseijden, Eric Farrell, Johan W. van Neck, Stefan O.P. Hofer |
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Přispěvatelé: | Plastic and Reconstructive Surgery and Hand Surgery, Otorhinolaryngology and Head and Neck Surgery, Orthopedics and Sports Medicine |
Rok vydání: | 2010 |
Předmět: |
Stromal cell
Angiogenesis Biomedical Engineering Adipose tissue Mice Nude lcsh:Medicine Mice Tissue engineering Fatty acid binding Spheroids Cellular Animals Humans Vimentin Stem cell transplantation for articular cartilage repair Cell Proliferation Transplantation Tissue Engineering Chemistry Mesenchymal stem cell lcsh:R Endothelial Cells Cell Biology Flow Cytometry Immunohistochemistry Coculture Techniques Cell biology Endothelial stem cell Platelet Endothelial Cell Adhesion Molecule-1 Adipose Tissue Endothelium Vascular Stromal Cells |
Zdroj: | Cell Transplantation, Vol 19 (2010) Cell Transplantation, 19(8), 1007-1020. SAGE Publishing |
ISSN: | 0963-6897 |
Popis: | Vascularization is still one of the most important limitations for the survival of engineered tissues after implantation. In this study, we aim to improve the in vivo vascularization of engineered adipose tissue by preforming vascular structures within in vitro-engineered adipose tissue constructs that can integrate with the host vascular system upon implantation. Different cell culture media were tested and different amounts of human adipose tissue-derived mesenchymal stromal cells (ASC) and human umbilical vein endothelial cells (HUVEC) were combined in spheroid cocultures to obtain optimal conditions for the generation of prevascularized adipose tissue constructs. Immunohistochemistry revealed that prevascular structures were formed in the constructs only when 20% ASC and 80% HUVEC were combined and cultured in a 1:1 mixture of endothelial cell medium and adipogenic medium. Moreover, the ASC in these constructs accumulated lipid and expressed the adipocyte-specific gene fatty acid binding protein-4. Implantation of prevascularized ASC/HUVEC constructs in nude mice resulted in a significantly higher amount of vessels (37 ± 17 vessels/mm2) within the constructs compared to non-prevascularized constructs composed only of ASC (3 ± 4 vessels/mm2). Moreover, a subset of the preformed human vascular structures (3.6 ± 4.2 structures/mm2) anastomosed with the mouse vasculature as indicated by the presence of intravascular red blood cells. Our results indicate that preformed vascular structures within in vitro-engineered adipose tissue constructs can integrate with the host vascular system and improve the vascularization upon implantation. |
Databáze: | OpenAIRE |
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