The phagocytic fitness of leucopatches may impact the healing of chronic wounds

Autor:	Hannah Trøstrup, Kim Thomsen, Rasmus Lundquist, Claus Moser, Niels Høiby, Lars Christophersen
Rok vydání:	2016
Předmět:	Adult Male 0301 basic medicine Neutrophils Phagocytosis Primary Cell Culture Immunology medicine.disease_cause Models Biological Pathogenesis 030207 dermatology & venereal diseases 03 medical and health sciences 0302 clinical medicine Cell Movement medicine Humans Immunologic Factors Immunology and Allergy In Situ Hybridization Respiratory Burst Wound Healing Migration Assay Platelet-Rich Plasma Pseudomonas aeruginosa business.industry Original Articles Middle Aged Healthy Volunteers Respiratory burst 030104 developmental biology Biofilms Platelet-rich plasma Adjunctive treatment Female Reactive Oxygen Species Wound healing business
Zdroj:	Clinical and Experimental Immunology. 184:368-377
ISSN:	1365-2249 0009-9104
DOI:	10.1111/cei.12773
Popis:	Summary Chronic non-healing wounds are significantly bothersome to patients and can result in severe complications. In addition, they are increasing in numbers, and a challenging problem to the health-care system. Handling of chronic, non-healing wounds can be discouraging due to lack of improvement, and a recent explanation can be the involvement of biofilm infections in the pathogenesis of non-healing wounds. Therefore, new treatment alternatives to improve outcome are continuously sought-after. Autologous leucopatches are such a new, adjunctive treatment option, showing promising clinical effects. However, the beneficial effect of the patches are not understood fully, although a major contribution is believed to be from the release of stimulating growth factors from activated thrombocytes within the leucopatch. Because the leucopatches also contain substantial numbers of leucocytes, the aim of the present study was to investigate the activity of the polymorphonuclear neutrophils (PMNs) within the leucopatch. By means of burst assay, phagocytosis assay, migration assay, biofilm killing assay and fluorescence in-situ hybridization (FISH) assay we showed significant respiratory burst in PMNs, active phagocytosis and killing of Pseudomonas aeruginosa by the leucopatch. In addition, bacterial-induced migration of PMNs from the leucopatch was shown, as well as uptake of P. aeruginosa by PMNs within the leucopatch. The present study substantiated that at least part of the beneficial clinical effect in chronic wounds by leucopatches is attributed to the activity of the PMNs in the leucopatch.
Databáze:	OpenAIRE
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