A novel role of HuR in ‐Epigallocatechin‐3‐gallate (EGCG ) induces tumour cells apoptosis
| Autor: | Wei Liu, Lei Song, Yousheng Mo, Jiangang Shen, Wenxuan Jian, Sha Xiong, Jiansong Fang, Dongli Li, Shuhuan Fang, Qi Wang, Honghai Hong, Tongkai Chen, Yong Xia |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Amyloid MAP Kinase Signaling System Short Communication ADAM10 Short Communications Apoptosis PC12 Cells Catechin ELAV-Like Protein 1 Metastasis ADAM10 Protein Amyloid beta-Protein Precursor 03 medical and health sciences 0302 clinical medicine Antigen Neoplasms Neuroblastoma mental disorders medicine Animals Humans bcl-2-Associated X Protein Ribonucleoprotein Regulation of gene expression Chemistry Membrane Proteins Hep G2 Cells Cell Biology medicine.disease Rats Gene Expression Regulation Neoplastic 030104 developmental biology Proto-Oncogene Proteins c-bcl-2 030220 oncology & carcinogenesis Cancer research Molecular Medicine Amyloid Precursor Protein Secretases |
| Zdroj: | Journal of Cellular and Molecular Medicine |
| ISSN: | 1582-4934 1582-1838 |
| DOI: | 10.1111/jcmm.14249 |
| Popis: | Hepatocellular carcinoma (HCC) is one of the most common malignancies worldwide.1 Inducing tumour cells apoptosis by chemotherapy is a common treatment modality for inoperable tumour.2 Some studies have shown that amyloid protein precursor (APP), which plays an important role in neuronal cells owing to their linkage with Alzheimer's disease,3 aberrantly altered in many types of cancers. However, the regulation of APP in tumour cells and its role in tumour cells remains unknown.‐Epigallocatechin‐3‐gallate (EGCG) has been shown to possess a wide range of pharmacological properties, including anti‐inflammatory and neuroprotective effects, which have been linked to the antioxidant/pro‐oxidant properties of its polyphenol constituents.4 Some studies have shown that EGCG inhibited hepatocellular carcinoma growth and induced apoptosis.5 We previously also found that EGCG induced neuroblastoma apoptosis via inhibition of amyloid precursor protein.6 However, little is known about the mechanism of EGCG on APP regulation in tumour. The RNA‐binding proteins (RBPs) interact with other proteins and RNAs to form different ribonucleoprotein (RNP) complexes, which participate in the post‐transcriptional regulation of RNAs and which orchestrate the fate of those RNAs.7 Human antigen R (HuR, also known as ELAVL1) is an important RNA‐binding protein. Some studies reported that HuR correlated with patient outcome in liver cancer and interacted with lncRNA‐{"type":"entrez-nucleotide","attrs":{"text":"AK058003","term_id":"16554001","term_text":"AK058003"}}AK058003 to regulate proliferation and metastasis of hepatocellular carcinoma.7 Furthermore, in neurons, studies showed that HuD interacted with the 3’ UTRs of APP mRNA (encoding amyloid precursor protein) and BACE1 mRNA (encoding β‐site APP‐cleaving enzyme 1) and increased the half‐lives of these mRNAs.8 However, the role of HuR in APP expression and EGCG‐regulated APP expression in tumour is not clear. In this study, we first indicate that the role of EGCG in APP and ADAM10 regulation is mediated by HuR, result in tumour cells apoptosis via HuR‐Erk1/2‐APP/ADAM10 pathway, adding a new dimension to EGCG‐mediated regulation of APP and ADAM10 in cancer cell apoptosis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text