Evaluation of Tubulointerstitial Lesions' Severity in Patients with Glomerulonephritides: An NMR-Based Metabonomic Study
| Autor: | Sofia Dimou, Rigas Kalaitzidis, Nikolaos G. Psihogios, Eleni Bairaktari, Kostas C. Siamopoulos, Konstantin Seferiadis |
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| Rok vydání: | 2007 |
| Předmět: |
Male
Glycosuria medicine.medical_specialty Magnetic Resonance Spectroscopy Urinalysis Biopsy Urinary system Urology Renal function Acetates Kidney Biochemistry chemistry.chemical_compound Glomerulonephritis Internal medicine medicine Humans Lactic Acid Least-Squares Analysis Aged Creatinine medicine.diagnostic_test business.industry Kidney metabolism General Chemistry Middle Aged medicine.disease Metabolism Endocrinology chemistry Spectrophotometry Chemistry Clinical Aminoaciduria Female Renal biopsy medicine.symptom business |
| Zdroj: | Journal of Proteome Research. 6:3760-3770 |
| ISSN: | 1535-3907 1535-3893 |
| Popis: | An 1H NMR-based metabonomic approach was used to investigate the correlation of histopathologically assessed tubulointerstitial lesions with the urinary metabolite profile in 77 patients with glomerulonephritides submitted to renal biopsy. The presence of renal damage was predicted with a sensitivity of 96% and a specificity of 99%. Patients with mild, moderate, and severe tubulointerstitial lesions were progressively differentiated from the healthy individuals in the Orthogonal Signal Correction Partial Least-Squares-Discriminant Analysis (OSC/PLS-DA) models with a statistically significant separation between those with mild and with severe lesions. The onset of the tubulointerstitial lesions is characterized by decreased excretion of citrate, hippurate, glycine, and creatinine, whereas further deterioration is followed by glycosuria, selective aminoaciduria, total depletion of citrate and hippurate, and gradual increase in the excretion of lactate, acetate, and trimethylamine-N-oxide. NMR-based metabonomic urinalysis could contribute to the early evaluation of the severity of the renal damage and possibly to the monitoring of kidney function. |
| Databáze: | OpenAIRE |
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