Janus Particles as Artificial Antigen-Presenting Cells for T Cell Activation
| Autor: | Yilong Jia, Yan Yu, Lucero Sanchez, Stephen M. Anthony, Yuan Gao, Bo Chen |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Materials science
Immunological Synapses T-Lymphocytes T cell Intracellular Space Antigen-Presenting Cells Biocompatible Materials Janus particles Nanotechnology Lymphocyte Activation Jurkat cells Article Immunological synapse Jurkat Cells Artificial antigen presenting cells medicine Humans General Materials Science Antigen-presenting cell Artificial cell Models Immunological medicine.anatomical_structure Biophysics Artificial Cells Calcium Intracellular Biotechnology |
| Zdroj: | ACS Applied Materials & Interfaces. 6:18435-18439 |
| ISSN: | 1944-8252 1944-8244 |
| DOI: | 10.1021/am505510m |
| Popis: | Here we show that the multifunctionality of Janus particles can be exploited for in vitro T cell activation. We engineer bifunctional Janus particles on which the spatial distribution of two ligands, anti-CD3 and fibronectin, mimics the "bull's eye" protein pattern formed in the membrane junction between a T cell and an antigen-presenting cell. Different levels of T cell activation can be achieved by simply switching the spatial distribution of the two ligands on the surfaces of the "bull's eye" particles. We find that the ligand pattern also affects clustering of intracellular proteins. This study demonstrates that anisotropic particles, such as Janus particles, can be developed as artificial antigen-presenting cells for modulating T cell activation. |
| Databáze: | OpenAIRE |
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