Persistent Exposure to Environmental Levels of Microcystin-LR Disturbs Cortisol Production via Hypothalamic-Pituitary-Interrenal (HPI) Axis and Subsequently Liver Glucose Metabolism in Adult Male Zebrafish (Danio rerio)

Autor:	Li Li, Lingkai Wang, Liping Yang, Wang Lin, Dandan Zhang, Honghui Guo, Dapeng Li, Qingji Zha, Rong Tang
Rok vydání:	2020
Předmět:	Blood Glucose Male endocrine system Hypothalamo-Hypophyseal System medicine.medical_specialty animal structures Hydrocortisone Microcystins Health Toxicology and Mutagenesis Danio lcsh:Medicine Microcystin-LR Endocrine Disruptors cortisol 010501 environmental sciences Biology Carbohydrate metabolism Kidney Toxicology 01 natural sciences Article 03 medical and health sciences chemistry.chemical_compound Downregulation and upregulation Internal medicine medicine Animals Homeostasis Zebrafish 030304 developmental biology 0105 earth and related environmental sciences 0303 health sciences microcystin-LR lcsh:R Zebrafish Proteins zebrafish biology.organism_classification Endocrinology Gene Expression Regulation Liver Endocrine disruptor Nuclear receptor chemistry Marine Toxins hypothalamic-pituitary-interrenal axis liver glucose metabolism Phosphoenolpyruvate carboxykinase Water Pollutants Chemical
Zdroj:	Toxins Toxins, Vol 12, Iss 282, p 282 (2020) Volume 12 Issue 5
ISSN:	2072-6651
Popis:	There is growing evidence that microcystin-LR (MC-LR) is a new endocrine disruptor, whereas the impacts of persistent exposure to MC-LR on the hypothalamic-pituitary-interrenal (HPI) axis and health hazards thereafter have not been investigated. In this work, adult male zebrafish (Danio rerio) were immersed into MC-LR solutions at concentrations of 0, 1, 5 and 25 &mu g/L for 30 d, respectively. The results showed that persistent MC-LR exposure caused an extensive upregulation of HPI-axis genes but an inhibition of brain nuclear receptors (gr and mr), which finally increased serum cortisol levels. Furthermore, the decreased expression of hepatic gr might partly be responsible for the strong inhibition on the expression of downstream genes involved in glucose metabolic enzymes, including gluconeogenesis-related genes (pepck, fbp1a, g6pca), glycogenolysis-related gene (pyg), glycolysis-related genes (gk, pfk1b, pk) and glycogenesis-related gene (gys2). These findings are in accordance with the decline in serum glucose, indicating that long-term MC-LR exposure caused a lower production of glucose relative to glucose lysis. Our above results firstly establish the link between persistent MC-LR exposure and impaired glucose metabolism, suggesting that long-term MC-LR-mediated stress might threaten fish&rsquo s heath.
Databáze:	OpenAIRE
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