Expression of the cDNA encoding lipocortin-like 39 kDa protein of guinea pig neutrophils in yeast Purification and biological characterization
| Autor: | Masayasu Inoue, Hirotsugu Kobuchi, Seiji Morishita, Eisuke F. Sato, Keisuke Edashige, Yasuo M. Sugino, Kozo Utsumi, Yoshikazu Tanaka |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
Recombinant protein
Annexins Neutrophils Saccharomyces cerevisiae Genetic Vectors Guinea Pigs Biophysics Biology Lipocortin Biochemistry Protein kinase Structural Biology Complementary DNA HSPA2 Genetics Animals Cloning Molecular Protein kinase A Molecular Biology Expression vector Binding protein Calcium-Binding Proteins Neutrophil Cell Biology DNA biology.organism_classification Molecular biology Yeast Recombinant Proteins Molecular Weight Phospholipases Tyrosine kinase Plasmids |
| Zdroj: | FEBS Letters. (2):231-236 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/0014-5793(89)81097-X |
| Popis: | The cDNA encoding lipocortin-like 39 kDa protein in guinea pig neutrophils was cloned into a yeast expression vector and the constructed plasmid was introduced into a yeast. The gene was expressed in an eukaryotic cell, yeast Saccharomyces cerevisiae and the recombinant protein was purified and characterized. The purified protein was identical with the native one with respect to the antigenicity and several biochemical properties, such as inhibitory action against phospholipase A 2 , Ca 2+ -dependent binding to acidic-phospholipids and F-actin and availability as a substrate for tyrosine kinase (EGF receptor/kinase) and protein kinase C. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|