Knockdown of eukaryotic translation initiation factor 4E suppresses cell growth and invasion, and induces apoptosis and cell cycle arrest in a human lung adenocarcinoma cell line
| Autor: | Chengchu Zhu, Jian Zhang, Bo Zhang, Yu Chen, Baofu Chen, Quanteng Hu, Lilong Xia |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: |
eukaryotic translation initiation factor 4E
Cancer Research Cell cycle checkpoint Lung Neoplasms Cell Adenocarcinoma of Lung Apoptosis Biology Adenocarcinoma Transfection Biochemistry Small hairpin RNA RNA interference Cell Line Tumor Genetics medicine Humans Neoplasm Invasiveness RNA Messenger RNA Small Interfering Molecular Biology Cell Proliferation A549 cell Gene knockdown Cell growth EIF4E Articles Cell Cycle Checkpoints Cell cycle cell invasion Molecular biology Cell biology medicine.anatomical_structure Eukaryotic Initiation Factor-4E Oncology Cell culture Gene Knockdown Techniques Cancer research Molecular Medicine cell cycle Corrigendum A431 cells |
| Zdroj: | Molecular Medicine Reports |
| ISSN: | 1791-3004 1791-2997 |
| Popis: | Eukaryotic translation initiation factor 4E (eIF4E) was shown to be upregulated in malignant human tumors. To assess the effect of downregulation of eIF4E on the prolifera- tion and invasiveness of a human lung adenocarcinoma cell line, a short hairpin (sh)RNA targeting eIF4E was constructed and transfected into A549 human lung adenocarcinoma cells. The expression of eIF4E was determined by reverse tran- scription-quantitative polymerase chain reaction and western blotting. Cell viability was assessed using a Cell Counting kit-8, and apoptosis levels and cell cycle distribution were assessed by flow cytometry. Invasiveness was assessed using Transwell chambers. Transfection of the A549 cells with eIF4E targeting shRNA reduced the mRNA and protein expression levels of eIF4E by >70% 48 and 72 h following transfection, and eIF4E targeting shRNA-transfected cells were significantly less viable compared with the cells transfected with scrambled shRNA. The rate of apoptosis was also significantly increased, significantly more cells were in the G 0/G1 phase and fewer were in the S phase, indicating cell cycle arrest. The fraction of transfected cells migrating across Transwell inserts were also reduced. In conclusion, inhibition of eIF4E suppressed cell growth and invasion, induced apoptosis and cell cycle arrest, suggesting that eIF4E may be a potential therapeutic target in lung adenocarcinoma. |
| Databáze: | OpenAIRE |
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