Effects of combination therapy using basic fibroblast growth factor and mature adipocyte-derived dedifferentiated fat (DFAT) cells on skin graft revascularisation

Autor:	Kosuke Morioka, Kazutaka Soejima, Taro Matsumoto, Hiroaki Nakazawa, Takashi Asami, Tomohiko Kazama, Tsutomu Kashimura
Rok vydání:	2015
Předmět:	medicine.medical_specialty Combination therapy Angiogenesis medicine.medical_treatment Basic fibroblast growth factor Neovascularization Physiologic Rats Sprague-Dawley chemistry.chemical_compound Adipocyte medicine Adipocytes Animals Humans Regeneration Progenitor cell Cells Cultured Wound Healing integumentary system business.industry Mesenchymal stem cell Dermis Skin Transplantation Cell Dedifferentiation Surgery chemistry Models Animal Cancer research Skin grafting Fibroblast Growth Factor 2 Wound healing business
Zdroj:	Journal of plastic surgery and hand surgery. 49(4)
ISSN:	2000-6764
Popis:	Although the benefits of basic fibroblast growth factor (bFGF) for wound healing and angiogenesis are well known, its effects on the process of skin graft revascularisation have not been clarified. It was hypothesised that bFGF would be beneficial to promote taking of skin grafts, but that the effect might be limited in the case of bFGF monotherapy. Therefore, this study investigated the efficacy of combination therapy using bFGF and dedifferentiated fat (DFAT) cells. DFAT cells have multilineage differentiation potential, including into endothelial cells, similar to the case of mesenchymal stem cells (MSC).Commercially available human recombinant bFGF was used. DFAT cells were prepared from SD strain rats as an adipocyte progenitor cell line from mature adipocytes. Full-thickness skin was lifted from the back of SD strain rats and then grafted back to the original wound site. Four groups were established prior to skin grafting: control group (skin graft alone), bFGF group (treated with bFGF), DFAT group (treated with DFAT cells), and combination group (treated with both bFGF and DFAT cells). Tissue specimens for histological examination were harvested 48 hours after grafting.The histological findings for the bFGF group showed vascular augmentation in the grafted dermis compared with the control group. However, the difference in the number of revascularised vessels per unit area did not reach statistical significance against the control group. In contrast, in the combination group, skin graft revascularisation was significantly promoted, especially in the upper dermis.The results suggest that replacement of the existing graft vessels was markedly promoted by the combination therapy using bFGF and DFAT cells, which may facilitate skin graft taking.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::5f7fd7ba8bc3cd6640919758bda51ae6 https://pubmed.ncbi.nlm.nih.gov/25744232 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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