Tubgcp3 is a mitotic regulator of planarian epidermal differentiation
| Autor: | Cui Ge, Shoutao Zhang, Cun Lin, Xiyan Feng, Zhitai Hao, Qingnan Tian, Mengzhen Zhang, Jingchen Yuan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
03 medical and health sciences 0302 clinical medicine Genetics Animals Regeneration Cloning Molecular Mitosis Tissue homeostasis Cell Proliferation biology Regeneration (biology) Cell Differentiation General Medicine Helminth Proteins Planarians biology.organism_classification Cell biology Spindle apparatus 030104 developmental biology Centrosome Planarian 030220 oncology & carcinogenesis Dugesia japonica Stem cell Epidermis Microtubule-Associated Proteins |
| Zdroj: | Gene. 775 |
| ISSN: | 1879-0038 |
| Popis: | Tubgcp3/GCP3 (The centrosomal protein γ-tubulin complex protein 3) is a component of the γ-tubulin small complexes (γ-TuSCs) and γ-tubulin ring complexes (γ-TuRCs), which play critical roles in mitotic spindle formation during mitosis. However, its function in stem cell development has not been thoroughly elucidated. The planarian flatworm, which contains a large number of adult somatic stem cells (neoblasts), is a unique model to study stem cell lineage development in vivo. Here, we identified a homolog of Tubgcp3 in planarian Dugesia japonica, and found that Tubgcp3 is required for the maintenance of epidermal lineage. RNAi targeting Tubgcp3 resulted in tissue homeostasis and regeneration defect. Knockdown of Tubgcp3 reduced cell divisions and led to a loss of the mature epidermal cells. Our findings indicate that Tubgcp3 is a mitotic regulator and plays a crucial role in planarian epidermal differentiation. |
| Databáze: | OpenAIRE |
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