Development of a peptide aptamer pair-linked rapid fluorescent diagnostic system for Zika virus detection
| Autor: | Bao Tuan Duong, Seon-Ju Yeo, Hyun Park, Anh Thi Viet Nguyen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Aptamer
In silico Biomedical Engineering Biophysics Peptide Biosensing Techniques Dengue virus Cross Reactions medicine.disease_cause Antibodies Viral Zika virus Dengue Antigen Electrochemistry medicine Humans chemistry.chemical_classification Rapid diagnostic test biology Zika Virus Infection General Medicine Zika Virus biology.organism_classification Virology Fluorescence chemistry Peptides Aptamers Peptide Biotechnology |
| Zdroj: | Biosensorsbioelectronics. 197 |
| ISSN: | 1873-4235 |
| Popis: | A rapid diagnostic system employing an antigen detection biosensing method is needed to discriminate between Zika virus (ZIKV) and Dengue virus (DENV) due to their close antigenic homology. We developed a novel peptide pair-based flow immunochromatographic test strip (FICT) assay to detect ZIKV. Peptide aptamers, P6.1 (KQERNNWPLTWT), P29.1 (KYTTSTLKSGV), and B2.33 (KRHVWVSLSYSCAEA) were designed by paratopes and modified against the ZIKV envelope protein based on the binding affinity. An antibody-free lateral FICT was developed using a pair of peptide aptamers. In the rapid diagnostic strip, the limit of detection (LOD) for the B2.33-P6.1 peptide pair for ZIKV was 2 × 104 tissue culture infective dose TCID50/mL. Significantly, FICT could discriminate ZIKV from DENV. The stability and performance of FICT were confirmed using human sera and urine, showing a comparable LOD value. Our study demonstrated that in silico modeling could be used to develop a novel peptide pair-based FICT assay for detecting ZIKV by a rapid diagnostic test. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect