New Secale cereale (rye) DNA derivatives for the detection of rye chromosome segments in wheat
| Autor: | C. L. McIntyre, S. Pereira, Rudi Appels, L. B. Moran |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Secale
clone (Java method) Molecular Sequence Data Molecular cloning Chromosomes Genetics Repeated sequence Molecular Biology Triticum Repetitive Sequences Nucleic Acid biology digestive oral and skin physiology Nucleic acid sequence Nucleic Acid Hybridization food and beverages Chromosome DNA General Medicine biology.organism_classification Molecular biology Clone Cells Subcloning Genetic marker Karyotyping DNA Probes Biotechnology |
| Zdroj: | Genome. 33:635-640 |
| ISSN: | 1480-3321 0831-2796 |
| Popis: | Subcloning of a clone of the 120-bp family of rye, pSc119, has produced two extremely useful probes. pSc119.1 assays rye-specific dispersed repetitive sequence families. It is present on all seven rye chromosomes and hybridizes to the entire length of each chromosome, with the exception of some telomeres and the nucleolar organiser region. pSc119.2, in contrast, hybridizes predominantly to the telomeric regions of rye chromosomes, with some interstitial sites. Unlike pSc119.1, it assays similar repetitive sequence families in both wheat and rye chromosomes.Key words: Secale cereale, rye DNA probes, rye chromosome detection. |
| Databáze: | OpenAIRE |
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