Low-conductance high selective inositol (1,4,5)-trisphosphate activated Ca2+ channels in plasma membrane of A431 carcinoma cells
| Autor: | Svetlana B. Semyonova, Galina N. Mozhayeva, A G Mamin, K I Kiselyov |
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| Rok vydání: | 1997 |
| Předmět: |
Patch-Clamp Techniques
Biophysics Inositol 1 4 5-Trisphosphate Biochemistry Membrane Potentials chemistry.chemical_compound Structural Biology Genetics Tumor Cells Cultured Humans Inositol Molecular Biology Membrane potential Chemistry Calcium channel Cell Membrane T-type calcium channel Electric Conductivity Cell Biology Hyperpolarization (biology) Inositol trisphosphate receptor Phosphatidylinositol 4 5-bisphosphate A431 cell Inositol (1 4 5)-trisphosphate Calcium Channels Intracellular Plasma membrane |
| Zdroj: | FEBS letters. 407(3) |
| ISSN: | 0014-5793 |
| Popis: | In many cells, activation of receptors coupled to PIP2 turnover results in Ca2+ release from the intracellular stores accompanied by Ca2+ influx across the PM. It is not well established yet whether Ca2+ influx is activated by IP3 or by an unknown signal generated upon Ca2+ store depletion. We report here a single-channel study of low-conductance IP3-activated channels of very high selectivity for Ca2+ in the PM of A431 carcinoma cells. The channels are strongly potential dependent and sensitive to [Ca2+]i within the physiological range. The data obtained argues for IP3 acting directly on plasma membrane Ca2+ channels. |
| Databáze: | OpenAIRE |
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