L4 stage Heligmosomoides polygyrus prevents the maturation of dendritic JAWS II cells
Autor: | Maria Doligalska, Marta Maruszewska-Cheruiyot, Katarzyna Krawczak, Karolina Piechna, Katarzyna Donskow-Łysoniewska |
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Rok vydání: | 2018 |
Předmět: |
Male
Immunology Population Apoptosis Bone Marrow Cells Proinflammatory cytokine Cell Line Mice Sex Factors Animals education CD86 education.field_of_study Nematospiroides dubius biology Cell Cycle NF-kappa B General Medicine Dendritic cell Dendritic Cells Cell cycle biology.organism_classification Endocytosis Cell biology Specific Pathogen-Free Organisms Mice Inbred C57BL Infectious Diseases Cell culture Larva Cytokines Parasitology Female Heligmosomoides polygyrus Chemokines CD80 |
Zdroj: | Experimental parasitology. 196 |
ISSN: | 1090-2449 |
Popis: | Helminths and their products are strong candidates for the treatment of autoimmunological disorders and allergies. Being a key population of antigen-presenting cells, dendritic cells play a crucial role in the therapeutic potential of worms. The study compares the effects of live pre-male and pre-female L4 stage Heligmosomoides polygyrus administration on the maturation and activation of the JAWS II line of immature dendritic cells. On stimulation with L4 stage H. polygyrus, JAWS II cells acquire semi-mature status and induce Th2 and regulatory responses in vitro. The strongest immunosuppressive effect on JAWS II cells was observed following stimulation with both sexes of nematodes together; this was manifested as immature dendritic cell morphology, proliferation inhibition, cell cycle change, decreased translocation of NF-κB into the nucleus, and lower expression of surface cellular costimulatory molecules CD80, CD86 and MHC I. However, greater production of proinflammatory (IL-12p70, TNF-α, IL-6) and Th2 response-promoting cytokines (IL-4) was observed by JAWS II following exposure to both sexes compared to male or female larvae alone. Sex had no influence on the viability, apoptosis process or endocytosis abilities of the JAWS II cell line. The findings indicate that the presence of only a single sex of the parasite influences a developed response, resulting in reduced proinflammatory and an antiparasitic reaction. |
Databáze: | OpenAIRE |
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