Chemical profiling of Curatella americana Linn leaves by UPLC-HRMS and its wound healing activity in mice

Autor: Cleydson B. R. Santos, Dayse Maria Cunha Sá, Francisco Fábio Oliveira de Sousa, Washington Luiz Assunção Pereira, Milton N. da Silva, José Adolfo Homobono Machado Bittencourt, Carolina Miranda de Sousa Lima, C. Silva, Jocivania Oliveira da Silva, Abraão de Jesus Barbosa Muribeca, Mayara Amoras Teles Fujishima
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Leaves
Physiology
Plant Science
Pathology and Laboratory Medicine
Cardiovascular Physiology
High-performance liquid chromatography
Biochemistry
Catechin
chemistry.chemical_compound
Oxidative Damage
Mice
0302 clinical medicine
Anti-Infective Agents
Glucosides
Animal Cells
Tandem Mass Spectrometry
Dilleniaceae
Medicine and Health Sciences
Medicine
Immune Response
Connective Tissue Cells
0303 health sciences
Multidisciplinary
Traditional medicine
integumentary system
Plant Anatomy
Antimicrobial
Bacterial Pathogens
Connective Tissue
Medical Microbiology
030220 oncology & carcinogenesis
Quercetin
Cellular Types
Anatomy
Pathogens
Brazil
Research Article
Curatella americana
Science
Immunology
Microbiology
03 medical and health sciences
Signs and Symptoms
Glucoside
Diagnostic Medicine
Tissue Repair
Animals
Humans
Kaempferols
Microbial Pathogens
030304 developmental biology
Inflammation
Flavonoids
Wound Healing
business.industry
Plant Extracts
Biology and Life Sciences
Cell Biology
Fibroblasts
Plant Leaves
Biological Tissue
chemistry
Angiogenesis
Kaempferol
Wound healing
business
Physiological Processes
Reactive Oxygen Species
Developmental Biology
Chromatography
Liquid
Zdroj: PLoS ONE, Vol 15, Iss 1, p e0225514 (2020)
PLoS ONE
ISSN: 1932-6203
Popis: Based on ethnopharmacological studies, a lot of plants, as well as its compounds, have been investigated for the potential use as wound healing agents. In Brazil, Curatella americana is traditionally used by local people to treat wounds, ulcers and inflammations. However, to the best of our knowledge, its traditional use in the treatment of wounds has not been validated by a scientific study. Here, some compounds, many of them flavonoids, were identified in the hydroethanolic extract from the leaves of C. americana (HECA) by LC-HRMS and LC-MS/MS. Besides that, solutions containing different concentrations of HECA and a gel produced with this extract were evaluated for its antimicrobial, coagulant and wound healing activities on an excision mouse wound model as well as its acute dermal safety. A total of thirteen compounds were identified in HECA, mainly quercetin, kaempferol and glucoside derivatives of both, besides catechin and epicatechin known as wound healing agents. The group treated with 1% of HECA exhibited highest wound healing activity and best rate of wound contraction confirmed by histopathology results. The present study provides scientific evidence of, this extract (HECA) possess remarkable wound healing activity, thereby, supporting the traditional use.
Databáze: OpenAIRE
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