High-Resolution Mapping of a Fruit Firmness-Related Quantitative Trait Locus in Tomato Reveals Epistatic Interactions Associated with a Complex Combinatorial Locus
| Autor: | Guiping Sun, Peter Glen Walley, Mathilde Causse, Julien Bonnet, Neil S. Graham, Mervin Poole, Laurent Grivet, Graham B. Seymour, Rebecca Smith, James R. Lynn, Charles Baxter, Graham J.W. King, Natalie H. Chapman |
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| Přispěvatelé: | University of Nottingham, UK (UON), Syngenta France, Appl Stat Solut, Partenaires INRAE, Sch Life Sci, La Trobe University, Campden BRI, Génétique et Amélioration des Fruits et Légumes (GAFL), Institut National de la Recherche Agronomique (INRA), Southern Cross University (SCU), Syngenta Ltd, UK Biotechnology and Biological Sciences Research Council [BB/D00103X, BB/G02491X], Syngenta, European Solanaceae Integrated Project EUSOL [Food-CT-2006-016214] |
| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Candidate gene Physiology [SDV]Life Sciences [q-bio] Quantitative Trait Loci Population INTROGRESSION LINES TEXTURE Locus (genetics) Plant Science Biology Quantitative trait locus 01 natural sciences Chromosomes Plant CANDIDATE GENES PENNELLII 03 medical and health sciences Solanum lycopersicum Gene mapping Gene Expression Regulation Plant Genetics Genomics and Molecular Evolution Genetics [SDV.BV]Life Sciences [q-bio]/Vegetal Biology education Base Pairing Gene Genetic Association Studies GENE-EXPRESSION 030304 developmental biology Recombination Genetic 2. Zero hunger 0303 health sciences education.field_of_study Models Genetic LYCOPERSICON-ESCULENTUM BACKCROSS QTL ANALYSIS Chromosome Mapping food and beverages Epistasis Genetic ETHYLENE Phenotype Genetic marker Fruit FRESH-MARKET TOMATO Epistasis RIPENING-INHIBITOR 010606 plant biology & botany |
| Zdroj: | Plant Physiology Plant Physiology, American Society of Plant Biologists, 2012, 159 (4), pp.1644-1657. ⟨10.1104/pp.112.200634⟩ |
| ISSN: | 1532-2548 0032-0889 |
| Popis: | Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; Fruit firmness in tomato (Solanum lycopersicum) is determined by a number of factors including cell wall structure, turgor, and cuticle properties. Firmness is a complex polygenic trait involving the coregulation of many genes and has proved especially challenging to unravel. In this study, a quantitative trait locus (QTL) for fruit firmness was mapped to tomato chromosome 2 using the Zamir Solanum pennellii interspecific introgression lines (ILs) and fine-mapped in a population consisting of 7,500 F2 and F3 lines from IL 2-3 and IL 2-4. This firmness QTL contained five distinct subpeaks, Fir(s.p.)QTL2.1 to Fir(s.p.)QTL2.5, and an effect on a distal region of IL 2-4 that was nonoverlapping with IL 2-3. All these effects were located within an 8.6-Mb region. Using genetic markers, each subpeak within this combinatorial locus was mapped to a physical location within the genome, and an ethylene response factor (ERF) underlying Fir(s.p.)QTL2.2 and a region containing three pectin methylesterase (PME) genes underlying Fir(s.p.)QTL2.5 were nominated as QTL candidate genes. Statistical models used to explain the observed variability between lines indicated that these candidates and the nonoverlapping portion of IL 2-4 were sufficient to account for the majority of the fruit firmness effects. Quantitative reverse transcription-polymerase chain reaction was used to quantify the expression of each candidate gene. ERF showed increased expression associated with soft fruit texture in the mapping population. In contrast, PME expression was tightly linked with firm fruit texture. Analysis of a range of recombinant lines revealed evidence for an epistatic interaction that was associated with this combinatorial locus. |
| Databáze: | OpenAIRE |
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