Certification procedures used by the Biological Stain Commission for eriochrome cyanine R (C.I. 43820, Mordant blue 3)
| Autor: | John A. Kiernan, C Fagan |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Chromoxane cyanine R Histology Erythrocytes Iron Eriochrome cyanine R Solochrome cyanine R 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Biological stain Cyanine Coloring Agents Hematoxylin Myelin Sheath Cell Nucleus 030102 biochemistry & molecular biology Chemistry Benzenesulfonates Histological Techniques Mordant General Medicine Hydrogen-Ion Concentration Medical Laboratory Technology 030220 oncology & carcinogenesis Nuclear chemistry |
| Zdroj: | Biotechnichistochemistry : official publication of the Biological Stain Commission. 95(5) |
| ISSN: | 1473-7760 |
| Popis: | Eriochrome cyanine R (C.I. 43820, Mordant blue 3), also known as chromoxane cyanine R and solochrome cyanine R, has been used as a biological stain since 1957. In conjunction with ferric ions, it provides selective blue coloration of the nuclei of cells in methods procedurally similar to commonly used progressive or regressive hemalum (aluminum-hematoxylin) stains. Eriochrome cyanine R also is used to stain the myelin sheaths of axons in nerve tissue; the results are visually similar to those in sections stained with luxol fast blue MBS (C.I. 74180, solvent blue 38) with selective blue coloration of myelin and erythrocytes. Eriochrome cyanine R is an article of commerce with many uses in industrial coloration and analytical chemistry; it can be used instead of either hematoxylin or luxol fast blue MBS, especially in the event of a shortage of either of the latter compounds. The Biological Stain Commission (BSC) will certify batches of eriochrome cyanine R that meet the criteria set out in this document. The criteria include satisfactory UV/visible spectra at pH 4 and pH 12 - 13, a dye content not less than 40% and not greater than 52% (calculated as the color acid; equivalent to 46 - 59% of the trisodium salt), and satisfactory performance in three staining methods: regressive for nuclei, progressive for nuclei and regressive for myelin. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |