Isolation of Lineage-Restricted Neuronal Precursors from Multipotent Neuroepithelial Stem Cells
| Autor: | Anjali J. Kalyani, Margot Mayer-Pröschel, Mahendra S. Rao, Tahmina Mujtaba |
|---|---|
| Rok vydání: | 1997 |
| Předmět: |
Neuroscience(all)
Cellular differentiation Retinoic acid Neural Cell Adhesion Molecule L1 Biology Fibroblast growth factor Rats Sprague-Dawley chemistry.chemical_compound Tubulin Glial Fibrillary Acidic Protein Animals Progenitor cell Neural Cell Adhesion Molecules Cells Cultured Progenitor Neurons Stem Cells General Neuroscience fungi Cell Differentiation Epithelial Cells Embryo Mammalian Clone Cells Rats Cell biology Neuroepithelial cell Oligodendroglia Spinal Cord nervous system chemistry Astrocytes Sialic Acids Neural cell adhesion molecule Stem cell Neuroglia Neuroscience |
| Zdroj: | Neuron. 19:773-785 |
| ISSN: | 0896-6273 |
| Popis: | We have identified a neuronal-restricted precursor (NRP) cell that expresses E-NCAM (high polysialic-acid NCAM) and is morphologically distinct from multipotent neuroepithelial (NEP) cells (Kalyani et al. 1997) and spinal glial progenitors (Rao and Mayer-Proschel 1997). NRP cells self renew over multiple passages in the presence of fibroblast growth factor (FGF) and neurotrophin-3 (NT-3) and differentiate in the presence of retinoic acid and the absence of FGF into postmitotic neurons. NRP cells can also be generated from multipotent E10.5 NEP cells. Clonal analysis shows that NRP cells arise from a NEP progenitor that generates other restricted CNS precursors. The NEP-derived NRPs undergo self renewal and can differentiate into multiple neuronal phenotypes. Thus, a direct lineal relationship exists between multipotential NEP cells and more restricted neuronal precursor cells present in vivo at E13.5 in the spinal cord. |
| Databáze: | OpenAIRE |
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