Humoral and mucosal IgA antibody response to a recombinant 52-kDa cysteine-rich portion of the Entamoeba histolytica galactose-inhibitable lectin correlates with detection of native 170-kDa lectin antigen in serum of patients with amebic colitis
| Autor: | Ismael Abou-El-Magd, Jonathan I. Ravdin, Ahmed M. El-Hawey, Chu-Jing Ginny Soong |
|---|---|
| Rok vydání: | 1996 |
| Předmět: |
Immunoglobulin A
Saliva Antibodies Protozoan Antigens Protozoan Enzyme-Linked Immunosorbent Assay Immunoglobulin G Microbiology Entamoeba histolytica Feces Antigen Lectins medicine Immunology and Allergy Animals Humans Colitis Intestinal Mucosa biology Galactose medicine.disease biology.organism_classification Recombinant Proteins Infectious Diseases Case-Control Studies Humoral immunity Immunology biology.protein Antibody |
| Zdroj: | The Journal of infectious diseases. 174(1) |
| ISSN: | 0022-1899 |
| Popis: | Humoral and mucosal IgA responses to a recombinant cysteine-rich portion (designated LC3) of the Entamoeba histolytica galactose-inhibitable lectin's 170-kDa subunit were determined in patients with amebic colitis. All patients had 170-kDa amebic antigen in serum, compared with 1 of 50 cyst passers and 1 of 31 controls (P < .01). Seven days after treatment, serum and fecal 170-kDa antigen became undetectable in 12 of the 13 patients (P < .001). Serum anti-LC3 IgA was found in 83.8% of colitis patients, compared with 2% of controls and 12% of asymptomatic cyst passers (P < .001). Salivary and fecal anti-LC3 IgA levels were higher in patients than in cyst passers (P < .001). In conclusion, in amebic colitis, development of humoral and mucosal IgA responses to the recombinant LC3-encoded protein correlates with detection of amebic 170-kDa antigen in serum and feces. |
| Databáze: | OpenAIRE |
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