Lentinan relieves hepatitis B surface antigen induced functional impairment of monocytes/macrophages
| Autor: | Jie Li, Le Qin, Shanshan Huang, Peipei Fang, Chenwei Pan, Lu Zhuge, Lingxiang Jin, Guangyao Zhou |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Hepatitis B virus MAPK/ERK pathway HBsAg Necrosis Lipopolysaccharide Lentinan Pharmaceutical Science medicine.disease_cause Molecular biology Blot 03 medical and health sciences chemistry.chemical_compound 030104 developmental biology chemistry Cell culture medicine Pharmacology (medical) medicine.symptom Hepatitis B surface antigen Lentinan Immuno-suppression Pro-inflammatory Antiinflammatory |
| Zdroj: | Tropical Journal of Pharmaceutical Research; Vol 17, No 4 (2018); 583-588 |
| ISSN: | 1596-5996 1596-9827 |
| Popis: | Purpose : To investigate the efficacy of lentinan in relieving hepatitis B surface antigen (HBsAg)- induced functional impairment of monocytes/macrophages. Methods : Monocytic cell line THP-1 was incubated with lentinan and HBsAg for 24 h and then stimulated with LPS (Lipopolysaccharide). The expression levels of interleukin-1β (IL-1β), IL-12 and tumour necrosis factor-α (TNF-α) were measured by enzyme-linked immunosorbent (ELISA) assays and quantitative reverse transcriptase-PCR (q-PCR). Protein levels of IkB-α, phospho-ERK, and phospho-p38 were measured by western blotting. Results : THP-1 cells treated with lentinan and HBsAg showed higher IL-1β, IL-12, and TNF-α levels than cells treated with HBsAg alone. The underlying mechanisms were associated with NF-kB and MAPK signal pathways. Decreased expression of IkB-α and phospho-ERK and increased expression of phospho-JNK and phospho-p38 were observed in cells treated with lentinan and HBsAg when compared with cells treated with HBsAg alone (p < 0.001). THP-1 cells incubated with 500 μg/mL lentinan secreted lower levels of cytokines than did control cells after LPS stimulation, suggesting an anti-inflammatory effect for lentinan. Conclusion : Lentinan shows both pro- and anti-inflammatory functions and may be a promising candidate for hepatitis B virus (HBV) treatment. Keywords : Hepatitis B surface antigen, Lentinan, Immuno-suppression, Pro-inflammatory, Antiinflammatory |
| Databáze: | OpenAIRE |
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