Structurally Specific Heparan Sulfates Support Primitive Human Hematopoiesis by Formation of a Multimolecular Stem Cell Niche
| Autor: | Joseph J. Brazil, Theodore R. Oegema, Pankaj Gupta, Arkadiusz Z. Dudek, Arne Slungaard, Catherine M. Verfaillie |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Stromal cell
Cellular differentiation Immunology Antigens CD34 Bone Marrow Cells Biology Biochemistry Extracellular matrix Cell Adhesion Humans Progenitor cell Cells Cultured Glycosaminoglycans Interleukin 3 Extracellular Matrix Proteins Molecular Structure Sulfates Cell Biology Hematology Hematopoietic Stem Cells Hematopoiesis Cell biology Molecular Weight Haematopoiesis Cell culture Culture Media Conditioned Cytokines Proteoglycans Heparitin Sulfate Stem cell Heparan Sulfate Proteoglycans Protein Binding |
| Zdroj: | Scopus-Elsevier |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v92.12.4641.424k10_4641_4651 |
| Popis: | Stem cell localization, conservation, and differentiation is believed to occur in niches in the marrow stromal microenvironment. Our recent observation that long-term in vitro human hematopoiesis requires a stromal heparan sulfate proteoglycan (HSPG) led us to hypothesize that such HSPG may orchestrate the formation of the stem cell niche. We compared the structure and function of HS from M2-10B4, a hematopoiesis-supportive cell line, with HS from a nonsupportive cell line, FHS-173-We. Long-term culture-initiating cell (LTC-IC) maintenance was enhanced by PG from supportive cells but not by PG from nonsupportive cells (P |
| Databáze: | OpenAIRE |
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