miR‑149 promotes the myocardial differentiation of mouse bone marrow stem cells by targeting Dab2
| Autor: | Shanghui Yi, Tao Chen, Nan Su, Tao Guo, Fuquan Luo, Min Wang, Lingling Xu, Mingjun Lu |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cancer Research Gene Expression Biology Biochemistry Cell Line 03 medical and health sciences Mice 0302 clinical medicine microRNA Genetics Animals Humans Myocytes Cardiac Molecular Biology Wnt Signaling Pathway Cells Cultured Adaptor Proteins Signal Transducing Oncogene Mesenchymal stem cell Wnt signaling pathway Bone Marrow Stem Cell Cell Differentiation Mesenchymal Stem Cells Cell biology Adaptor Proteins Vesicular Transport MicroRNAs 030104 developmental biology Real-time polymerase chain reaction Oncology Gene Expression Regulation 030220 oncology & carcinogenesis Molecular Medicine Ectopic expression RNA Interference Signal transduction Apoptosis Regulatory Proteins Biomarkers |
| Zdroj: | Molecular medicine reports. 17(6) |
| ISSN: | 1791-3004 |
| Popis: | To investigate the role of microRNA (miR)‑149 in the cardiac differentiation of mouse bone marrow mesenchymal stem cells (MSCs) in vitro, MSCs were infected with a lentivirus overexpressing miR‑149 and the effect on cardiac differentiation was determined. The quantitative polymerase chain reaction results demonstrated that miR‑149 promoted the expression of cardiac‑specific markers in MSCs. Western blotting and a luciferase activity assay demonstrated that disabled homolog 2 (Dab2) was a direct target of miR‑149. Dab2 ectopic expression and Wnt/β‑catenin signaling pathway inhibition was able to reverse the increased expression of cardiac‑specific markers induced by miR‑149. In conclusion, miR‑149 was able to target Dab2 and promote the cardiac differentiation of mouse MSCs in vitro, which depended upon the Wnt/β‑catenin signaling pathway. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|