Myotube-derived exosomal miRNAs downregulate Sirtuin1 in myoblasts during muscle cell differentiation
| Autor: | Etienne Lefai, Audrey Jalabert, Hubert Vidal, Sophie Rome, Sandra Pesenti, Karim Chikh, Elizabeth Errazuriz, Vanessa Euthine, Alexis Forterre, Aurélie Granjon |
|---|---|
| Přispěvatelé: | Cardiovasculaire, métabolisme, diabétologie et nutrition (CarMeN), Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Institut National de la Recherche Agronomique (INRA), Fondation pour la Recherche Medicale (FRM ), Association Francaise de recherche sur les Myopathies (AFM ), Association Francaise de Diabetologie (SDF ), INRA-specific grant (ANSSD), Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National des Sciences Appliquées de Lyon (INSA Lyon), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Hospices Civils de Lyon (HCL)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hospices Civils de Lyon (HCL), Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université de Lyon-Institut National des Sciences Appliquées (INSA)-Université Claude Bernard Lyon 1 (UCBL) |
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
MicroRNAs/*biosynthesis/classification/genetics
[SDV]Life Sciences [q-bio] Muscle Fibers Skeletal Biology Skeletal/metabolism Exosomes Exosome Muscle Fibers Myoblasts 03 medical and health sciences Mice 0302 clinical medicine Report medicine Cell Differentiation/*genetics Sirtuins Myocyte Animals Homeostasis Sirtuins/*biosynthesis/genetics Developmental Muscle Skeletal Molecular Biology 030304 developmental biology 0303 health sciences Myogenesis Muscle cell differentiation Wnt signaling pathway Gene Expression Regulation Developmental Skeletal muscle Cell Differentiation Cell Biology Microvesicles Cell biology MicroRNAs Exosomes/metabolism medicine.anatomical_structure Gene Expression Regulation 030220 oncology & carcinogenesis Muscle Myoblasts/cytology/*metabolism Skeletal/growth & development/metabolism C2C12 Developmental Biology |
| Zdroj: | Cell Cycle Cell Cycle, Taylor & Francis, 2014, 13 (1), pp.78-89. ⟨10.4161/cc.26808⟩ |
| ISSN: | 1538-4101 1551-4005 |
| DOI: | 10.4161/cc.26808⟩ |
| Popis: | International audience; It has recently been established that exosomes can mediate intercellular cross-talk under normal and pathological conditions through the transfer of specific miRNAs. As muscle cells secrete exosomes, we addressed the question of whether skeletal muscle (SkM) exosomes contained specific miRNAs, and whether they could act as "endocrine signals" during myogenesis. We compared the miRNA repertoires found in exosomes released from C2C12 myoblasts and myotubes and found that 171 and 182 miRNAs were exported into exosomes from myoblasts and myotubes, respectively. Interestingly, some miRNAs were expressed at higher levels in exosomes than in their donor cells and vice versa, indicating a selectivity in the incorporation of miRNAs into exosomes. Moreover miRNAs from C2C12 exosomes were regulated during myogenesis. The predicted target genes of regulated exosomal miRNAs are mainly involved in the control of important signaling pathways for muscle cell differentiation (e.g., Wnt signaling pathway). We demonstrated that exosomes from myotubes can transfer small RNAs (C. elegans miRNAs and siRNA) into myoblasts. Moreover, we present evidence that exosome miRNAs secreted by myotubes are functionally able to silence Sirt1 in myoblasts. As Sirt1 regulates muscle gene expression and differentiation, our results show that myotube-exosome miRNAs could contribute to the commitment of myoblasts in the process of differentiation. Until now, myokines in muscle cell secretome provided a conceptual basis for communication between muscles. Here, we show that miRNA exosomal transfer would be a powerful means by which gene expression is orchestrated to regulate SkM metabolic homeostasis. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|