Isolation of a cDNA for the catalytic subunit of rat liver glucose-6-phosphatase: regulation of gene expression in FAO hepatoma cells by insulin, dexamethasone and cAMP
| Autor: | M R el-Maghrabi, S J Pilkis, Wansong Pan, Alex J. Lange, S. R. Maitra, D. Argaud |
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| Rok vydání: | 1994 |
| Předmět: |
DNA
Complementary medicine.medical_treatment Phosphatase Molecular Sequence Data Biophysics Gene Expression Biology Biochemistry Dexamethasone Liver Neoplasms Experimental Complementary DNA medicine Cyclic AMP Animals Insulin Northern blot Amino Acid Sequence RNA Messenger RNA Neoplasm Molecular Biology Peptide sequence DNA Primers Regulation of gene expression Messenger RNA Base Sequence Sequence Homology Amino Acid cDNA library Cell Biology Molecular biology Rats Liver Glucose-6-Phosphatase Sequence Alignment |
| Zdroj: | Biochemical and biophysical research communications. 201(1) |
| ISSN: | 0006-291X |
| Popis: | cDNA clones coding for the catalytic subunit of rat liver glucose-6-phosphatase (EC 3.1.3.9) were isolated from a rat liver cDNA library in lambda gt11 phage. The sequence of the cDNA and the amino acid sequence derived from it were greater than 90% identical to the corresponding sequences for the mouse and human forms of liver glucose-6-phosphatase. Northern blot analysis of RNA from FAO hepatoma cells revealed that dexamethasone induced the glucose-6-phosphatase mRNA while insulin suppressed its expression. When both hormones were added together insulin completely suppressed the effect of glucocorticoid. cAMP addition alone decreased the abundance of glucose-6-phosphatase mRNA. The results demonstrate multihormonal regulation of gene expression of hepatic glucose-6-phosphatase and support a dominant role for insulin. |
| Databáze: | OpenAIRE |
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