The Microsporum canis genome is organized into five chromosomes based on evidence from electrophoretic karyotyping and chromosome end mapping
| Autor: | Jeny R. Cursino-Santos, Antonio Rossi, Gabriela F. Persinoti, Luciene M. Coelho, Nilce Maria Martinez-Rossi |
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| Rok vydání: | 2012 |
| Předmět: |
Genome
Genome Size Microsporum Microsporum canis DNA Fungal Genome size Southern blot Genetics Endodeoxyribonucleases biology Fungal genetics Chromosome Chromosome Mapping Karyotype MAPEAMENTO CROMOSSÔMICO General Medicine Telomere biology.organism_classification Molecular biology DNA Fingerprinting Electrophoresis Gel Pulsed-Field Blotting Southern Infectious Diseases DNA profiling Karyotyping Chromosomes Fungal Genome Fungal |
| Zdroj: | Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual) Universidade de São Paulo (USP) instacron:USP |
| ISSN: | 1460-2709 |
| Popis: | The karyotype of Microsporum canis was analyzed by contoured-clamped homogeneous electric field (CHEF) gel electrophoresis. Four chromosomal bands that correspond to five chromosomes ranging from 3.0-6.2 Mb were identified, adding the total genome size to approximately 24.9 Mb. To confirm the number of chromosomes in M. canis, the number of telomeres was assessed by using a telomeric probe (TTAGGG)(4) in Southern blot analyses of digested genomic DNA. Treatment of M. canis DNA with Bal31 exonuclease revealed progressive shortening of the DNA fragments positive for the (TTAGGG)(4) sequence, supporting location of repeats at the chromosome ends. These results can aid in improving the understanding of the genetic characterization of M. canis and the molecular epidemiology of dermatophytoses caused by this fungus. |
| Databáze: | OpenAIRE |
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